Abstract | BACKGROUND: Sensitization to house dust mite allergens is strongly correlated with asthma. Der p 7 elicits strong IgE antibody and T-cell responses in patients with mite allergy. However, the structure and biological function of this important allergen are unknown. Allergen function might contribute to allergenicity, as shown for the protease activity of group 1 mite allergens and the interaction with the innate immune system by group 2 mite allergens. OBJECTIVE: We sought to determine the crystal structure of Der p 7 and to investigate its biological function. METHODS: X-ray crystallography was used to determine the Der p 7 structure. Nuclear magnetic resonance analysis and biochemical assays were used to examine the binding of Der p 7 to predicted ligands. RESULTS: CONCLUSIONS:
Der p 7 binds a bacterially derived lipid product, a common feature of some allergens. The finding that the group 7, as well as the group 2, mite allergens are structurally similar to different proteins in the Toll-like receptor pathway further strengthens the connections between dust mites, innate immunity, and allergy.
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Authors | Geoffrey A Mueller, Lori L Edwards, Jim J Aloor, Michael B Fessler, Jill Glesner, Anna Pomés, Martin D Chapman, Robert E London, Lars C Pedersen |
Journal | The Journal of allergy and clinical immunology
(J Allergy Clin Immunol)
Vol. 125
Issue 4
Pg. 909-917.e4
(Apr 2010)
ISSN: 1097-6825 [Electronic] United States |
PMID | 20226507
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, N.I.H., Intramural)
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Copyright | Copyright (c) 2010 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved. |
Chemical References |
- Acute-Phase Proteins
- Antigens, Dermatophagoides
- Arthropod Proteins
- Carrier Proteins
- Dermatophagoides pteronyssinus antigen p 7
- Dust
- Membrane Glycoproteins
- Toll-Like Receptor 4
- lipopolysaccharide-binding protein
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Topics |
- Acute-Phase Proteins
(chemistry, metabolism)
- Animals
- Antigens, Dermatophagoides
(chemistry, immunology, metabolism)
- Arthropod Proteins
- Asthma
(etiology, immunology)
- Carrier Proteins
(chemistry, metabolism)
- Crystallography, X-Ray
- Dust
(immunology)
- Humans
- Hypersensitivity, Immediate
(etiology, immunology)
- Immunity, Innate
(immunology)
- Magnetic Resonance Spectroscopy
- Membrane Glycoproteins
(chemistry, metabolism)
- Mites
(immunology)
- Toll-Like Receptor 4
(chemistry, metabolism)
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