Abstract |
We improved medial collateral ligament (MCL) healing throughout 90 days after surgical transection. We introduced intraperitoneal, per-oral (in drinking water) and topical (thin cream layer) peptide therapy always given alone, without a carrier. Previously, as an effective peptide therapy, stable gastric pentadecapeptide BPC 157 (GEPPPGKPADDAGLV, an anti- ulcer peptide effective in inflammatory bowel disease therapy (PL 14736)) particularly improved healing of transected tendon and muscle and wound healing effect including the expression of the early growth response 1 (egr-1) gene. After MCL transection BPC 157 was effective in rats when given once daily intraperitoneally (10 microg or 10 ng/kg) or locally as a thin layer (1.0 microg dissolved in distilled water/g commercial neutral cream) at the site of injury, first application 30 min after surgery and the final application 24 h before sacrifice. Likewise, BPC 157 was effective given per-orally (0.16 microg/ml in the drinking water (12 ml/day/rat)) until sacrifice. Commonly, BPC 157 microg-ng-rats exhibited consistent functional, biomechanical, macroscopic and histological healing improvements. Thus, we suggest BPC 157 improved healing of acute ligament injuries in further ligament therapy.
|
Authors | Tomislav Cerovecki, Ivan Bojanic, Luka Brcic, Bozo Radic, Ivan Vukoja, Sven Seiwerth, Predrag Sikiric |
Journal | Journal of orthopaedic research : official publication of the Orthopaedic Research Society
(J Orthop Res)
Vol. 28
Issue 9
Pg. 1155-61
(Sep 2010)
ISSN: 1554-527X [Electronic] United States |
PMID | 20225319
(Publication Type: Journal Article)
|
Copyright | (c) 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. |
Chemical References |
- Collagen Type I
- Collagen Type III
- Peptide Fragments
- Proteins
- BPC 157
|
Topics |
- Administration, Oral
- Animals
- Collagen Type I
(metabolism)
- Collagen Type III
(metabolism)
- Contracture
(prevention & control)
- Disease Models, Animal
- Male
- Medial Collateral Ligament, Knee
(drug effects, injuries, physiology)
- Motor Activity
(drug effects)
- Neutrophils
(physiology)
- Peptide Fragments
(pharmacology)
- Proteins
(pharmacology)
- Rats
- Rats, Wistar
- Recovery of Function
(drug effects)
- Wound Healing
(drug effects)
|