[Production of interleukin-8 by circulating CLA+ T cells with skin tropism in patients with psoriasis and in healthy controls].

Abstract

BACKGROUND:

Psoriasis is an immune-mediated disease typically associated with cutaneous neutrophilic infiltration and Munro microabscesses. Interleukin (IL)-8 is one of the main neutrophil-attracting chemokines. Although keratinocytes have traditionally been considered to be the principal source of IL-8 in psoriasis, we present data that suggest that cutaneous lymphocyte associated antigen (CLA) + T lymphocytes synthesize this cytokine.

MATERIAL AND METHODS:

Six patients with psoriasis and 6 healthy controls were studied. Immunomagnetic separation was used to isolate CLA+ and CLA- T lymphocytes and IL-8 and interferon (IFN)-gamma production was quantified for each cell subpopulation using enzyme-linked immunosorbent assay. Finally, gene expression of IL-8 was analyzed by reverse transcriptase-polymerase chain reaction.

RESULTS:

CLA+ and CLA- T lymphocytes from patients with psoriasis and from controls showed a significantly increased production of IFN-gamma when activated, whereas only activated CLA+ T lymphocytes (from patients and controls) synthesized IL-8. The higher level of expression of IL-8 and IFN-gamma by CLA+T lymphocytes in comparison to CLA- cells was confirmed.

DISCUSSION:

Previous studies have confirmed IL-8 production by T lymphocytes in inflammatory skin diseases with neutrophil-rich infiltrates, such as acute generalized exanthematous pustulosis, Behçet disease, and pustular psoriasis. We have confirmed the role of the subset of T lymphocytes with skin tropism (CLA+) in IL-8 production in nonpustular psoriasis.

• Authors: M Ferran, M Ferran, A B Galván, A Giménez-Arnau, A Giménez-Arnau, R M Pujol, R M Pujol, L F Santamaría-Babi, L F Santamaría-Babi
• Journal: Actas dermo-sifiliograficas (Actas Dermosifiliogr) Vol. 101 Issue 2 Pg. 151-5 (Mar 2010) ISSN: 1578-2190 [Electronic] Spain
• Vernacular Title: Producción preferente de IL-8 por linfocitos T CLA+ circulantes con tropismo cutáneo en pacientes con psoriasis y en sujetos sanos.
• PMID: 20223157 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Differentiation, T-Lymphocyte
• Antigens, Neoplasm
• CTAGE1 protein, human
• Interleukin-8
• Membrane Glycoproteins
• Interferon-gamma

Topics

• Antigens, Differentiation, T-Lymphocyte (analysis)
• Antigens, Neoplasm (analysis)
• Chemotaxis
• Computer Systems
• Gene Expression Regulation
• Humans
• Immunologic Memory
• Interferon-gamma (biosynthesis, genetics)
• Interleukin-8 (biosynthesis, genetics)
• Lymphocyte Activation
• Membrane Glycoproteins (analysis)
• Neutrophils (immunology, physiology)
• Psoriasis (blood, immunology)
• Reverse Transcriptase Polymerase Chain Reaction
• Skin (immunology)
• T-Lymphocyte Subsets (chemistry, metabolism, physiology)