Abstract |
HIV-infected individuals have a significantly increased risk of developing an aggressive B-cell Non-Hodgkin Lymphoma relative to HIV(-) persons. Due to their aggressive nature, AIDS-related lymphomas (ARL) can also be more difficult to classify. Genetic abnormalities are known to play a significant role in HIV(-) lymphomagenesis. To aid in case classification and identify key pathogenetic events in ARL, we analyzed gene expression data and somatic DNA copy number changes by high-resolution array comparative genomic hybridization in tumors from 20 B-cell derived ARL (B-ARL) patients. Gene expression-based predictors robustly classified the B-ARL cases, distinguishing Burkitt lymphoma and diffuse large B-cell lymphoma, and identifying activated B-cell like and germinal center B-cell like molecular subtypes of diffuse large B-cell lymphoma. Array comparative genomic hybridization analysis revealed 13 recurrent losses and 16 recurrent gains in the B-ARL cases, including gain of 19p13.2 and loss of 16q23, not previously reported in B-ARL. The WWOX tumor suppressor gene was characterized as a candidate gene for the 16q23.1 locus and showed gene silencing or truncated transcript in 9 of 16 cases. These data demonstrate the ability to molecularly classify B-ARL lymphomas by gene expression and identified DNA copy number alterations targeted in B-ARL.
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Authors | Karen E Deffenbacher, Javeed Iqbal, Zhongfeng Liu, Kai Fu, Wing C Chan |
Journal | Journal of acquired immune deficiency syndromes (1999)
(J Acquir Immune Defic Syndr)
Vol. 54
Issue 1
Pg. 18-26
(May 01 2010)
ISSN: 1944-7884 [Electronic] United States |
PMID | 20216076
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Topics |
- Adult
- Burkitt Lymphoma
(diagnosis, pathology)
- Chromosome Aberrations
- Comparative Genomic Hybridization
- Diagnosis, Differential
- Female
- Gene Dosage
- Gene Expression
- HIV Infections
(complications)
- Humans
- Lymphoma, AIDS-Related
(diagnosis, pathology)
- Lymphoma, Large B-Cell, Diffuse
(diagnosis, pathology)
- Male
- Middle Aged
- Young Adult
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