HGF-induced invasion by prostate tumor cells requires anterograde lysosome trafficking and activity of Na+-H+ exchangers.

Abstract

Hepatocyte growth factor (HGF) is found in tumor microenvironments, and interaction with its tyrosine kinase receptor Met triggers cell invasion and metastasis. It was previously shown that acidic extracellular pH stimulated peripheral lysosome trafficking, resulting in increased cathepsin B secretion and tumor cell invasion, which was dependent upon sodium-proton exchanger (NHE) activity. We now demonstrate that HGF induced the trafficking of lysosomes to the cell periphery, independent of HGF-induced epithelial-mesenchymal transition. HGF-induced anterograde lysosome trafficking depended upon the PI3K pathway, microtubules and RhoA, resulting in increased cathepsin B secretion and invasion by the cells. HGF-induced NHE activity via increased net acid production, and inhibition of NHE activity with 5-(N-ethyl-N-isopropyl)-amiloride (EIPA), or a combination of the NHE1-specific drug cariporide and the NHE3-specific drug s3226 prevented HGF-induced anterograde trafficking and induced retrograde trafficking in HGF-overexpressing cells. EIPA treatment reduced cathepsin B secretion and HGF-induced invasion by the tumor cells. Lysosomes were located more peripherally in Rab7-shRNA-expressing cells and these cells were more invasive than control cells. Overexpression of the Rab7 effector protein, RILP, resulted in a juxtanuclear location of lysosomes and reduced HGF-induced invasion. Together, these results suggest that the location of lysosomes is an inherently important aspect of invasion by tumor cells.

• Authors: Joshua J Steffan, Brittany C Williams, Tomas Welbourne, James A Cardelli
• Journal: Journal of cell science (J Cell Sci) Vol. 123 Issue Pt 7 Pg. 1151-9 (Apr 01 2010) ISSN: 1477-9137 [Electronic] England
• PMID: 20215403 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• HGF protein, human
• Proton Pump Inhibitors
• Sodium-Hydrogen Exchangers
• rab7 GTP-Binding Proteins
• rab7 GTP-binding proteins, human
• Hepatocyte Growth Factor
• Cathepsin B
• rab GTP-Binding Proteins

Topics

• Cathepsin B (metabolism)
• Cell Line, Tumor
• Cell Movement (drug effects, genetics)
• Cloning, Molecular
• Cytoplasmic Streaming (drug effects, genetics)
• Hepatocyte Growth Factor (genetics, metabolism)
• Humans
• Lysosomes (metabolism)
• Male
• Neoplasm Invasiveness
• Phosphatidylinositol 3-Kinases (metabolism)
• Prostatic Neoplasms (metabolism, pathology)
• Proton Pump Inhibitors (pharmacology)
• Signal Transduction (drug effects, genetics)
• Sodium-Hydrogen Exchangers (antagonists & inhibitors)
• Transgenes (genetics)
• rab GTP-Binding Proteins (genetics, metabolism)
• rab7 GTP-Binding Proteins