Abstract |
Treatment of human immunodeficiency virus ( HIV) infection with highly active antiretroviral therapy ( HAART) is effective but can be associated with toxic effects and is expensive. Other options may be useful for long-term therapy. The immunomodulatory antibiotic minocycline could be an effective, low-cost adjunctive treatment to HAART. Minocycline mediated a dose-dependent decrease in single-cycle CXCR4-tropic HIV infection and decreased viral RNA after infection of CD4+ T cells with HIV NL4-3. Reactivation from latency was also decreased in a primary CD4+ T cell-derived model and in resting CD4+ T cells from HIV-infected patients. Minocycline treatment resulted in significant changes in activation marker expression and inhibited proliferation and cytokine secretion of CD4+ T cells in response to activation. This study demonstrates that minocycline reduces HIV replication and reactivation and decreases CD4+ T cell activation. The anti-HIV effects of minocycline are mediated by altering the cellular environment rather than directly targeting virus, placing minocycline in the class of anticellular anti-HIV drugs.
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Authors | Gregory L Szeto, Angela K Brice, Hung-Chih Yang, Sheila A Barber, Robert F Siliciano, Janice E Clements |
Journal | The Journal of infectious diseases
(J Infect Dis)
Vol. 201
Issue 8
Pg. 1132-40
(Apr 15 2010)
ISSN: 1537-6613 [Electronic] United States |
PMID | 20205570
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Anti-HIV Agents
- Cytokines
- DNA, Viral
- RNA, Viral
- Minocycline
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Topics |
- Anti-HIV Agents
(pharmacology, therapeutic use)
- CD4-Positive T-Lymphocytes
(chemistry, drug effects)
- Cell Transformation, Viral
(drug effects)
- Cells, Cultured
- Cytokines
(biosynthesis)
- DNA, Viral
(analysis)
- Dose-Response Relationship, Drug
- Enzyme-Linked Immunosorbent Assay
- HIV
(drug effects, physiology)
- HIV Infections
(drug therapy)
- Humans
- Lymphocyte Activation
(drug effects)
- Minocycline
(pharmacology, therapeutic use)
- RNA, Viral
(analysis)
- Reverse Transcriptase Polymerase Chain Reaction
- Viremia
(drug therapy, virology)
- Virus Latency
(drug effects)
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