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Science letters: Proteomic analysis of differentially expressed proteins in mice with concanavalin A-induced hepatitis.

AbstractOBJECTIVE:
To find new protein biomarkers for the detection and evaluation of liver injury and to analyze the relationship between such proteins and disease progression in concanavalin A (Con A)-induced hepatitis.
METHODS:
Twenty-five mice were randomly divided into five groups: an untreated group, a control group injected with phosphate buffered saline (PBS), and groups with Con A-induced hepatitis evaluated at 1, 3 and 6 h. Two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to identify differences in protein expression among groups. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to verify the results.
RESULTS:
In mice with Con A-induced hepatitis, expression levels of four proteins were increased: RIKEN, fructose bisphosphatase 1 (fbp1), ketohexokinase (khk), and Chain A of class pi glutathione S-transferase. Changes in fbp1 and khk were confirmed by qRT-PCR.
CONCLUSION:
Levels of two proteins, fbp1 and khk, are clearly up-regulated in mice with Con A-induced hepatitis.
AuthorsXu-fei Tan, Feng Chen, Shan-shan Wu, Yu Shi, Dong-cheng Liu, Zhi Chen
JournalJournal of Zhejiang University. Science. B (J Zhejiang Univ Sci B) Vol. 11 Issue 3 Pg. 221-6 (Mar 2010) ISSN: 1862-1783 [Electronic] China
PMID20205309 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Biomarkers
  • Proteome
  • Concanavalin A
Topics
  • Animals
  • Biomarkers (analysis)
  • Chemical and Drug Induced Liver Injury (etiology, metabolism)
  • Concanavalin A
  • Disease Models, Animal
  • Gene Expression Profiling
  • Gene Expression Regulation (drug effects)
  • Humans
  • Liver (drug effects, metabolism)
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Proteome (analysis)

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