Abstract | OBJECTIVE: METHODS: Twenty-five mice were randomly divided into five groups: an untreated group, a control group injected with phosphate buffered saline (PBS), and groups with Con A-induced hepatitis evaluated at 1, 3 and 6 h. Two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to identify differences in protein expression among groups. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to verify the results. RESULTS: CONCLUSION: Levels of two proteins, fbp1 and khk, are clearly up-regulated in mice with Con A-induced hepatitis.
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Authors | Xu-fei Tan, Feng Chen, Shan-shan Wu, Yu Shi, Dong-cheng Liu, Zhi Chen |
Journal | Journal of Zhejiang University. Science. B
(J Zhejiang Univ Sci B)
Vol. 11
Issue 3
Pg. 221-6
(Mar 2010)
ISSN: 1862-1783 [Electronic] China |
PMID | 20205309
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- Proteome
- Concanavalin A
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Topics |
- Animals
- Biomarkers
(analysis)
- Chemical and Drug Induced Liver Injury
(etiology, metabolism)
- Concanavalin A
- Disease Models, Animal
- Gene Expression Profiling
- Gene Expression Regulation
(drug effects)
- Humans
- Liver
(drug effects, metabolism)
- Male
- Mice
- Mice, Inbred BALB C
- Proteome
(analysis)
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