Detecting bacterial growth in continuous ambulatory peritoneal dialysis effluent using two culture methods.

Abstract

BACKGROUND/AIMS:

The aim of this study was to evaluate the peritonitis-causing bacteria detected in peritoneal fluid using a blood culture bottle in patients undergoing continuous ambulatory peritoneal dialysis (CAPD).

METHODS:

One-hundred and eleven dialysates from 43 patients suspected of peritonitis related to CAPD were retrospectively evaluated between May 2000 and February 2008. In all cases, 5 to 10 mL of dialysate was inoculated into a pair of BacT/Alert blood culture bottles, and 50 mL of centrifuged dialysate was simultaneously inoculated into a solid culture media for conventional culture. The results were compared to those of the conventional culture method. Isolated microorganisms were compared between the two methods.

RESULTS:

The blood culture method was positive in 78.6% (88 / 112) of dialysate specimens and the conventional culture method in 50% (56 / 112, p < 0.001).

CONCLUSIONS:

The blood culture method using the BacT/Alert system is useful for culturing dialysates and improves the positive culture rate in patients with suspected peritonitis compared to the conventional culture method.

• Authors: Se-Hee Yoon, Nak Won Choi, Sung-Ro Yun
• Journal: The Korean journal of internal medicine (Korean J Intern Med) Vol. 25 Issue 1 Pg. 82-5 (Mar 2010) ISSN: 1226-3303 [Print] Korea (South)
• PMID: 20195408 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Culture Media
• Dialysis Solutions

Topics

• Culture Media
• Dialysis Solutions
• Gram-Negative Bacterial Infections (diagnosis, microbiology)
• Gram-Positive Bacterial Infections (diagnosis, microbiology)
• Humans
• Kidney Failure, Chronic (therapy)
• Microbiological Techniques (methods)
• Peritoneal Dialysis, Continuous Ambulatory (adverse effects)
• Peritonitis (diagnosis, microbiology)
• Sensitivity and Specificity