Effects of ternary complexes of copper with salicylaldehyde-amino acid Schiff base coordination compounds on the proliferation of BGC823 cells.

Abstract

BACKGROUND AND OBJECTIVE:

The basic structure of salicylaldehyde-amino acid Schiff base compounds includes a C=N chemical bond. These compounds show significant antitumor activities in vitro when combined with a metal ion. This study investigated the effects and possible mechanisms of four salicylaldehyde-amino acid Schiff base copper ternary coordination compounds on the proliferation of human gastric cancer cell line BGC823.

METHODS:

The BGC823 cells were treated with the four compounds (6B, 7B, 6P, and 7P). Cell proliferation was detected by MTT assay. Apoptosis and changes in the cell cycle were analyzed by flow cytometry. DNA damage was observed using a DNA ladder assay. The expression of p53 protein was determined by immunocytochemistry.

RESULTS:

The proliferation of BGC823 cells was significantly inhibited by the four compounds and the effect was concentration-dependent. The half maximal inhibitory concentration (IC50) of 6B, 7B, 6P, and 7P for BGC823 cells were 18.10, 27.50, 3.61, and 3.45 micromol/L, respectively. Flow cytometry showed the four drugs induced apoptosis in BGC823 cells, which was confirmed by DNA ladder experiments. Flow cytometry also detected changed phases in the cell cycle from treatment with the compounds. The percent of cells in the G(0)/G(1) phase decreased and that of cells in the G1/S and G(2)/M phases increased, indicating that S-and G2-phase blockages exist. As shown by immunocytochemistry, the expression of p53 decreased in BGC823 cells treated with the four drugs, indicating the involvement of the p53 pathway to BGC823 cell apoptosis.

CONCLUSIONS:

The four compounds showed significant activities on restraining proliferation of BGC823 cells in vitro, induced apoptosis, and caused changes in the cell cycle. This may be related to the downregulation of p53.

• Authors: Ai-Juan Guo, Xiao-Shan Xu, Ying-Hui Hu, Ming-Zhao Wang, Xin Tan
• Journal: Chinese journal of cancer (Chin J Cancer) Vol. 29 Issue 3 Pg. 277-82 (Mar 2010) ISSN: 1000-467X [Print] England
• PMID: 20193110 (Publication Type: Journal Article)

Chemical References

• Aldehydes
• Amino Acids
• Antineoplastic Agents
• Coordination Complexes
• Schiff Bases
• Tumor Suppressor Protein p53
• salicylaldehyde
• Copper

Topics

• Aldehydes (chemistry)
• Amino Acids (chemistry)
• Antineoplastic Agents (chemical synthesis, pharmacology)
• Apoptosis (drug effects)
• Cell Cycle (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Coordination Complexes (chemical synthesis, pharmacology)
• Copper (chemistry)
• Humans
• Inhibitory Concentration 50
• Schiff Bases (chemistry)
• Stomach Neoplasms (metabolism, pathology)
• Tumor Suppressor Protein p53 (metabolism)