Cell uptake of Zn(II)-phthalocyanine-containing liposomes by clathrin-mediated endocytosis.

Abstract

The study of uptake mechanisms of therapeutic drugs has a growing interest in biomedical research. In this work the cell uptake and phototoxicity of the photosensitizer Zn(II)-phthalocyanine (ZnPc) in dipalmitoyl-phosphatidyl-choline liposomes have been studied in the presence or absence of inhibitors of macropinocytosis (cytochalasin D), and clathrin-mediated endocytosis (dynasore). No differences in the uptake or photodynamic damage were observed in A-549 cells subjected to incubation with either ZnPc alone or in combination with cytochalasin D. On the contrary, co-incubation of A-549 cells with ZnPc and dynasore resulted in a significant decrease of photodamage as well as negligible uptake of the photosensitizer. These results indicate that ZnPc is internalized into cells preferentially by a mechanism of clathrin-mediated endocytosis.

• Authors: Jorge Soriano, Juan C Stockert, Angeles Villanueva, Magdalena Cañete
• Journal: Histochemistry and cell biology (Histochem Cell Biol) Vol. 133 Issue 4 Pg. 449-54 (Apr 2010) ISSN: 1432-119X [Electronic] Germany
• PMID: 20191285 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Clathrin
• Indoles
• Isoindoles
• Liposomes
• Organometallic Compounds
• Photosensitizing Agents
• Zinc Compounds
• Zn(II)-phthalocyanine
• Cytochalasin D
• 1,2-Dipalmitoylphosphatidylcholine
• phthalocyanine

Topics

• 1,2-Dipalmitoylphosphatidylcholine (pharmacology)
• Biological Transport
• Cells (metabolism)
• Cellular Structures (metabolism)
• Clathrin (metabolism)
• Cytochalasin D (pharmacology)
• Endocytosis (drug effects)
• Indoles (metabolism)
• Isoindoles
• Liposomes (pharmacology)
• Organometallic Compounds (metabolism)
• Photosensitizing Agents (pharmacology)
• Zinc Compounds