Fluorescent quantum dot-labeled aptamer bioprobes specifically targeting mouse liver cancer cells.

Abstract

Fluorophore-labeled bioprobes are the key for fluorescent-labeled imaging technology. In the present work, mouse liver hepatoma cell line BNL 1ME A.7R.1 (MEAR)-specific ssDNA aptamer TLS9a was used to fabricate quantum dot-labeled aptamer bioprobe (QD-Apt), which was obtained by conjugating streptavidin-modified quantum dots (SA-QDs) with biotin-derived aptamer via the interaction between biotin and streptavidin. The QD-Apt was of monodispersity and excellent fluorescence properties. When the optimum ratio of SA-QDs to aptamer, which is 1:16, was used in the preparation of the QD-Apt, the resultant QD-Apt was of satisfactory bioactivity. They could specifically recognize MEAR cells and could not recognize BNL cells and Hela cells. Particularly, the growth and viability of QD-Apt bound MEAR cells were not affected by QD-Apt within 84 h compared to control cells, indicating that the probe was biocompatible and suitable for live cell imaging.

• Authors: Jing Zhang, Xing Jia, Xiao-Jing Lv, Yu-Lin Deng, Hai-Yan Xie
• Journal: Talanta (Talanta) Vol. 81 Issue 1-2 Pg. 505-9 (Apr 15 2010) ISSN: 1873-3573 [Electronic] Netherlands
• PMID: 20188954 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: (c) 2009 Elsevier B.V. All rights reserved.

Chemical References

• Aptamers, Nucleotide
• Biocompatible Materials
• Fluorescent Dyes
• Molecular Probes
• Biotin
• Streptavidin

Topics

• Animals
• Aptamers, Nucleotide (genetics, metabolism)
• Base Sequence
• Biocompatible Materials (chemistry, metabolism)
• Biotin (metabolism)
• Cell Line, Tumor
• Fluorescent Dyes (chemistry, metabolism)
• Kinetics
• Liver Neoplasms (metabolism, pathology)
• Mice
• Molecular Probes (chemistry, metabolism)
• Quantum Dots
• Reproducibility of Results
• Staining and Labeling
• Streptavidin (metabolism)
• Substrate Specificity
• Temperature