Rapid and reliable single nucleotide polymorphism-based differentiation of Brucella live vaccine strains from field strains.

Abstract

The reliable differentiation of live Brucella vaccine strains from field isolates is an important element in brucellosis control programs. We describe the design, validation, and implementation of a novel single nucleotide polymorphism (SNP)-based typing platform that offers a rapid, reliable, and robust tool to achieve this with improved diagnostic accuracy compared to existing molecular tests. Furthermore, the assays described are designed such that they supplement, and can be run as an intrinsic part of, a previously described assay identifying Brucella isolates to the species level (K. K. Gopaul, C. J. Smith, M. S. Koylass, and A. M. Whatmore, BMC Microbiol. 8:86), giving a comprehensive molecular typing platform.

• Authors: Krishna K Gopaul, Jessica Sells, Betsy J Bricker, Oswald R Crasta, Adrian M Whatmore
• Journal: Journal of clinical microbiology (J Clin Microbiol) Vol. 48 Issue 4 Pg. 1461-4 (Apr 2010) ISSN: 1098-660X [Electronic] United States
• PMID: 20181906 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Brucella Vaccine
• DNA, Bacterial

Topics

• Animals
• Bacterial Typing Techniques (methods)
• Brucella (classification, genetics)
• Brucella Vaccine (genetics)
• DNA Fingerprinting (methods)
• DNA, Bacterial (genetics)
• Genotype
• Humans
• Polymorphism, Single Nucleotide