Abstract |
The emergence in 1997 and continuance today of a highly lethal H5N1 avian influenza virus (AIV) causing human disease has raised concern about an impending pandemic and the need for a vaccine to prepare for such an occurrence. We previously generated an efficacious vesicular stomatitis virus (VSV)-based AIV vaccine expressing H5 hemagglutinin (HA) from the fifth genomic position of VSV (J. A. Schwartz et al., Virology 366:166-173, 2007). Here we have generated and characterized VSV-based vaccines that express the A/Hong Kong/156/1997 (clade 0) H5 HA from the first position of the VSV genome. These vectors induce broadly cross-neutralizing antibodies against homologous and heterologous H5N1 viruses of different clades in mice. The vaccines provide complete protection against morbidity and mortality after heterologous challenge with clade 0 and clade 1 strains in animals even 1 year after vaccination. Postchallenge pulmonary virus loads show that these vectors provide sterilizing immunity. Therefore, VSV-based AIV vaccines are potent, broadly cross-protective pandemic vaccine candidates.
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Authors | Jennifer A Schwartz, Linda Buonocore, Amorsolo L Suguitan Jr, Alex Silaghi, Darwyn Kobasa, Gary Kobinger, Heinz Feldmann, Kanta Subbarao, John K Rose |
Journal | Journal of virology
(J Virol)
Vol. 84
Issue 9
Pg. 4611-8
(May 2010)
ISSN: 1098-5514 [Electronic] United States |
PMID | 20181720
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, N.I.H., Intramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Neutralizing
- Antibodies, Viral
- Hemagglutinin Glycoproteins, Influenza Virus
- Influenza Vaccines
- hemagglutinin, avian influenza A virus
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Topics |
- Animals
- Antibodies, Neutralizing
(blood)
- Antibodies, Viral
(blood)
- Body Weight
- Cross Protection
- Female
- Genetic Vectors
- Hemagglutinin Glycoproteins, Influenza Virus
(genetics, immunology)
- Influenza A Virus, H5N1 Subtype
(immunology)
- Influenza Vaccines
(genetics, immunology)
- Lung
(virology)
- Mice
- Mice, Inbred BALB C
- Orthomyxoviridae Infections
(prevention & control, virology)
- Survival Analysis
- Time Factors
- Vesiculovirus
(genetics)
- Viral Plaque Assay
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