Abstract | BACKGROUND: Tumor initiating cells ( TICs) provide a new paradigm for developing original therapeutic strategies. METHODS: RESULTS: A long-term self-renewal capacity was particularly observed for cells of malignant glio-neuronal tumors (MGNTs). Cell sorting, karyotyping and proteomic analysis demonstrated cell stability throughout prolonged passages. Xenografts of fewer than 500 cells in Nude mouse brains induced a progressively growing tumor. CD133, CD15/LeX/ Ssea-1, CD34 expressions, or exclusion of Hoechst dye occurred in subsets of cells forming spheres, but was not predictive of their capacity to form secondary spheres or tumors, or to resist high doses of temozolomide. CONCLUSIONS:
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Authors | Cristina Patru, Luciana Romao, Pascale Varlet, Laure Coulombel, Eric Raponi, Josette Cadusseau, François Renault-Mihara, Cécile Thirant, Nadine Leonard, Alain Berhneim, Maria Mihalescu-Maingot, Jacques Haiech, Ivan Bièche, Vivaldo Moura-Neto, Catherine Daumas-Duport, Marie-Pierre Junier, Hervé Chneiweiss |
Journal | BMC cancer
(BMC Cancer)
Vol. 10
Pg. 66
(Feb 24 2010)
ISSN: 1471-2407 [Electronic] England |
PMID | 20181261
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- AC133 Antigen
- Antigens, CD
- Antigens, CD34
- Glycoproteins
- Lewis X Antigen
- PROM1 protein, human
- Peptides
- Prom1 protein, mouse
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Topics |
- AC133 Antigen
- Animals
- Antigens, CD
(biosynthesis)
- Antigens, CD34
(biosynthesis)
- Brain Neoplasms
(metabolism)
- Cell Line, Tumor
- Gene Expression Regulation, Neoplastic
- Glioma
(metabolism)
- Glycoproteins
(biosynthesis)
- Humans
- Lewis X Antigen
(biosynthesis)
- Mice
- Mice, Nude
- Neoplasm Transplantation
- Neoplastic Stem Cells
(cytology)
- Neurons
(pathology)
- Peptides
- Proteomics
(methods)
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