Abstract |
The development of T-cell responses in pigs vaccinated against Aujeszky's disease in the presence of maternal-derived antibodies (MDA) was examined. The aim of study was to evaluate the influence of MDA on the postvaccinal T-cell responses and optimization vaccination protocols in MDA-positive pigs. Pigs born to immune sows were vaccinated at different ages against Aujeszky's disease virus (ADV). For estimation of T-cell responses the lymphocyte proliferation and interferon (IFN)-gamma and interleukin-4 production were evaluated. High values of stimulation index were noted in groups vaccinated at 8 or 12 weeks of age (in 60% and 100% animals, respectively). In weaners vaccinated at 10 and 14 weeks of age, as well as in those vaccinated at 7 days and revaccinated at 8 or 12 weeks of age, 100% of animals positively responded in the lymphocyte proliferation assay after booster. At 20 weeks of life, only animals vaccinated at 12 weeks of age, 7 days and 12 weeks of age, and 10 and 14 weeks of age showed antigen-specific proliferation. Similar results were observed with IFN-gamma secretion after exposure to live ADV. We demonstrate that early vaccination with a live glycoprotein E-deleted ADV vaccine, in the face of high levels of MDA, could be effective, but the intensity and duration of the anamnestic response depends on the time of booster injection.
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Authors | Małgorzata Pomorska-Mól, Iwona Markowska-Daniel |
Journal | FEMS immunology and medical microbiology
(FEMS Immunol Med Microbiol)
Vol. 58
Issue 3
Pg. 405-11
(Apr 2010)
ISSN: 1574-695X [Electronic] England |
PMID | 20180849
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Viral
- Viral Vaccines
- Interferon-gamma
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Topics |
- Animals
- Antibodies, Viral
(immunology)
- Female
- Herpesvirus 1, Suid
(immunology)
- Immunity, Maternally-Acquired
(immunology)
- Interferon-gamma
(metabolism)
- Lymphocyte Activation
(immunology)
- Pregnancy
- Pseudorabies
(immunology, prevention & control)
- Swine
- Swine Diseases
(immunology, prevention & control)
- T-Lymphocytes
(immunology)
- Vaccination
- Viral Vaccines
(administration & dosage, immunology)
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