Abstract | BACKGROUND: METHODS: Diluted egg white proteins were separated by 2-dimensional (2-D) gel electrophoresis. Immunolabeling was performed on individual patient sera from 19 child patients with egg white allergy and 11 negative control subjects. Spots of egg white proteins that bound to the patients' IgE were identified by mass spectrometry-based proteomics. RESULTS: CONCLUSIONS: We demonstrated that L-PGDS and cystatin reacted with serum IgE in patients with egg allergy. Our proteomics-based analysis in egg white gives a comprehensive map of proteins bound with IgE and should assist in enabling more accurate diagnoses and recommendations of desensitizing treatments for individual patients.
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Authors | Makiko Suzuki, Hidehiko Fujii, Hidetsugu Fujigaki, Shinji Shinoda, Kanako Takahashi, Kuniaki Saito, Hisayasu Wada, Masumi Kimoto, Naomi Kondo, Mitsuru Seishima |
Journal | Allergology international : official journal of the Japanese Society of Allergology
(Allergol Int)
Vol. 59
Issue 2
Pg. 175-83
(Jun 2010)
ISSN: 1440-1592 [Electronic] England |
PMID | 20179418
(Publication Type: Journal Article)
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Chemical References |
- Allergens
- Cystatins
- Egg Proteins
- Lipocalins
- Recombinant Fusion Proteins
- Immunoglobulin E
- Intramolecular Oxidoreductases
- prostaglandin R2 D-isomerase
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Topics |
- Allergens
(chemistry, immunology)
- Blotting, Western
- Child
- Child, Preschool
- Cloning, Molecular
- Cystatins
(chemistry, immunology)
- Egg Hypersensitivity
(blood, immunology, physiopathology)
- Egg Proteins
(chemistry, immunology)
- Female
- Humans
- Immunoglobulin E
(blood, immunology)
- Infant
- Intramolecular Oxidoreductases
(chemistry, genetics, immunology, metabolism)
- Lipocalins
(chemistry, genetics, immunology, metabolism)
- Male
- Mass Spectrometry
- Proteomics
- Recombinant Fusion Proteins
(genetics)
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