Effect of indirubin-3-monoxime against lung cancer as evaluated by histological and transmission electron microscopic studies.

Abstract

The aim of this study is to evaluate the antitumor effect of indirubin-3-monoxime and its mode of action in benzo(α)pyrene [B(α)P] induced lung cancer in A/J mice. Light microscopic examination of lung sections of [B(α)P] induced lung cancer mice revealed the presence of adenocarcinoma characterized by extensive proliferation of alveolar epithelium and loss of alveolar spaces. The control lung tissue showed a normal architecture with clear alveolar spaces. Interestingly the indirubin-3-monoxime treated groups showed the reduced adenocarcinoma with appearance of alveolar spaces. Transmission Electron Microscopic (TEM) studies of lung sections of [B(α)P] induced lung cancer mice showed the presence of phaemorphic cells with dense granules and increased mitochondria. The lung sections of mice treated with indirubin-3-monoxime showed the presence of shrunken, fragmented, and condensed nuclei implying apoptosis. The effects were dose dependent and prominent in 10 mg/kg/5 d/week groups suggesting the therapeutic role of indirubin analogue against this deadly human malignancy. Here, our results indicate that indirubin-3-monoxime brings antitumor effect against [B(α)P] induced lung cancer by its apoptotic action in A/J mice.

• Authors: Kameswaran Ravichandran, Arttatrana Pal, Ramanibai Ravichandran
• Journal: Microscopy research and technique (Microsc Res Tech) Vol. 73 Issue 11 Pg. 1053-8 (Oct 2010) ISSN: 1097-0029 [Electronic] United States
• PMID: 20169620 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2010 Wiley-Liss, Inc.

Chemical References

• Antineoplastic Agents
• Indoles
• Oximes
• indirubin-3'-monoxime
• Benzo(a)pyrene

Topics

• Animals
• Antineoplastic Agents (pharmacology)
• Benzo(a)pyrene (toxicity)
• Cell Count
• Cell Nucleus (drug effects)
• Cell Proliferation (drug effects)
• Cell Shape (drug effects)
• Cytoplasm (drug effects)
• Histocytochemistry
• Indoles (pharmacology)
• Lung (anatomy & histology, cytology, pathology)
• Lung Neoplasms (chemically induced, pathology)
• Male
• Mice
• Microscopy, Electron, Transmission
• Oximes (pharmacology)
• Pulmonary Alveoli (cytology, drug effects)