Induction of apoptosis by [8]-shogaol via reactive oxygen species generation, glutathione depletion, and caspase activation in human leukemia cells.

Abstract

Ginger, the rhizome of Zingiber officinale , is a traditional medicine with a carminative effect and antinausea, anti-inflammatory, and anticarcinogenic properties. This study examined the growth inhibitory effects of [8]-shogaol, one of the pungent phenolic compounds in ginger, on human leukemia HL-60 cells. It demonstrated that [8]-shogaol was able to induce apoptosis in a time- and concentration-dependent manner. Treatment with [8]-shogaol caused a rapid loss of mitochondrial transmembrane potential, stimulation of reactive oxygen species (ROS) production, release of mitochondrial cytochrome c into cytosol, and subsequent induction of procaspase-9 and procaspase-3 processing. Taken together, these results suggest for the first time that ROS production and depletion of glutathione that contributed to [8]-shogaol-induced apoptosis in HL-60 cells.

• Authors: Po-Chuen Shieh, Yi-Own Chen, Daih-Huang Kuo, Fu-An Chen, Mei-Ling Tsai, Ing-Shing Chang, Hou Wu, Shengmin Sang, Chi-Tang Ho, Min-Hsiung Pan
• Journal: Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 58 Issue 6 Pg. 3847-54 (Mar 24 2010) ISSN: 1520-5118 [Electronic] United States
• PMID: 20163181 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Catechols
• Plant Extracts
• Reactive Oxygen Species
• shogaol
• Caspase 3
• Caspase 9
• Glutathione

Topics

• Apoptosis (drug effects)
• Caspase 3 (metabolism)
• Caspase 9 (metabolism)
• Catechols (pharmacology)
• Cell Line, Tumor
• Enzyme Activation (drug effects)
• Ginger (chemistry)
• Glutathione (metabolism)
• Humans
• Leukemia (enzymology, metabolism, physiopathology)
• Plant Extracts (pharmacology)
• Reactive Oxygen Species (metabolism)