The effects of different doses of
tylosin on serum
cytokine concentrations were investigated in healthy and
lipopolysaccharide-treated mice. The mice were divided into seven groups.
Lipopolysaccharide (LPS) was injected into the positive control group. The other six groups received three different
tylosin doses concurrently without or with LPS: 10 mg/kg, 100 mg/kg, 500 mg/kg, 10 mg/kg + LPS, 100 mg/kg + LPS and 500 mg/kg + LPS.
After treatment, serum samples were collected at 0, 1, 2, 3, 6, 12 and 24 hours. Serum tumour
necrosis factor alpha (
TNFalpha),
interleukin 1beta (IL1beta) and
IL10 levels were determined by
enzyme-linked
immunosorbent assay (ELISA).
Tylosin doses of 10 and 100 mg/kg induced no
cytokine production in the healthy mice.
Tylosin at 500 mg/kg had no effect on
TNFalpha or IL1beta production, but it induced
IL10 production in healthy mice. All doses of
tylosin reduced the elevated
TNFalpha and IL1beta in LPS-treated mice but increased their
IL10 levels. In conclusion, these data suggest that
tylosin has an immunomodulatory effect at the dose recommended for use against
infection.