Evaluation of erythrocyte band 3 phosphotyrosine level, glutathione content, CA-125, and human epididymal secretory protein E4 as combined parameters in endometriosis.

Abstract

OBJECTIVE:

To investigate the biochemical parameters of the erythrocyte response to diamide-induced oxidative stress, alone or as adjuncts to serum values of CA-125 and human epididymal secretory protein E4 (HE4), in the diagnosis and study of endometriosis.

SETTING:

University of Padova.

DESIGN:

Prospective study.

PATIENT(S):

Forty-five patients of reproductive age undergoing laparoscopy.

INTERVENTION(S):

All women were studied for endometriotic foci during laparoscopic surgery. Forty-one had laparoscopically and histologically confirmed endometriosis, and four did not. Twenty women with confirmed endometriosis were reassessed 1-4 months later.

MAIN OUTCOME MEASURE(S):

CA-125 and HE4 and two new parameters evaluated in erythrocytes after diamide-induced stress, that is, band 3 tyrosine phosphorylation (Tyr-P) level and decrease in total glutathione content (ΔGSH), were assessed in all patients.

RESULT(S):

In association with serum CA-125 levels but not with HE4, diamide-related erythrocyte band 3 Tyr-P and ΔGSH were significantly higher in patients with endometriosis and were able to discriminate with high sensitivity and specificity between patients before and after surgery.

CONCLUSION(S):

Endometriosis is associated with an increase in systemic oxidative stress, affecting the antioxidative defenses of circulating erythrocytes. All related implications, including evaluation of other oxidative stress-related changes, warrant further study.

• Authors: Luciana Bordin, Cristina Fiore, Gabriella Donà, Alessandra Andrisani, Guido Ambrosini, Diego Faggian, Mario Plebani, Giulio Clari, Decio Armanini
• Journal: Fertility and sterility (Fertil Steril) Vol. 94 Issue 5 Pg. 1616-21 (Oct 2010) ISSN: 1556-5653 [Electronic] United States
• PMID: 20153468 (Publication Type: Evaluation Study, Journal Article)
• Copyright: Copyright © 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

Chemical References

• Anion Exchange Protein 1, Erythrocyte
• Biomarkers
• CA-125 Antigen
• DEFB126 protein, human
• Epididymal Secretory Proteins
• beta-Defensins
• Phosphotyrosine
• Glutathione

Topics

• Adult
• Anion Exchange Protein 1, Erythrocyte
• Biomarkers (blood)
• CA-125 Antigen (blood)
• Case-Control Studies
• Endometriosis (blood, diagnosis)
• Epididymal Secretory Proteins (metabolism)
• Erythrocytes (metabolism)
• Female
• Glutathione (blood)
• Humans
• Oxidative Stress (physiology)
• Phosphotyrosine (blood)
• Prospective Studies
• Sensitivity and Specificity
• Uterine Diseases (blood, diagnosis)
• beta-Defensins