Crotonaldehyde induces oxidative stress and caspase-dependent apoptosis in human bronchial epithelial cells.
|
| Abstract |
Crotonaldehyde is a widespread environmental pollutant and lipid peroxidation product. Crotonaldehyde is a risk factor for many diseases (e.g., chronic pulmonary inflammation). However, its toxicity and its mechanism of action have not been thoroughly investigated. The purpose of this study is to investigate crotonaldehyde-induced oxidative stress and mechanism of cell death in BEAS-2B cells. Crotonaldehyde caused decreases of intracellular reduced glutathione levels and increases of reactive oxygen species in a dose-dependent manner. Crotonaldehyde induced cell death by apoptosis, and gradually transitioned to necrosis at high dose of crotonaldehyde, as demonstrated by Annexin V-FITC/PI staining and cell morphology analysis. Crotonaldehyde-induced ATP decline observed in the study might partially account for the switch from apoptosis to necrosis. Mitochondria membrane potential, cytochrome c release, caspase-9, and caspase-3/7 activity were investigated, and the results suggest that crotonaldehyde-induced apoptosis was activated in a caspase-dependent way. Collectively, these results demonstrate crotonaldehyde induces cell oxidative stress and caspase-dependent apoptosis.
|
|---|---|
| Authors | Xing-yu Liu, Zhi-hua Yang, Xiu-jie Pan, Mao-xiang Zhu, Jian-ping Xie |
| Journal | Toxicology letters (Toxicol Lett) Vol. 195 Issue 1 Pg. 90-8 (May 19 2010) ISSN: 1879-3169 [Electronic] Netherlands |
| PMID | 20153411 (Publication Type: Journal Article) |
| Copyright | Copyright (c) 2010 Elsevier Ireland Ltd. All rights reserved. |
| Chemical References |
|
| Topics |
|
Join CureHunter, for free Research Interface BASIC access!
Realize the full power of the drug-disease research graph!