Maintenance of glomerular filtration barrier integrity requires laminin alpha5.

Abstract

Mutation of the mouse laminin alpha5 gene results in a variety of developmental defects, including defects in kidney structure and function. Whereas the total absence of laminin alpha5 results in breakdown of the glomerular basement membrane (GBM) and failed glomerular vascularization, a hypomorphic Lama5 mutation (the Lama5(neo) allele) results in proteinuria, hematuria, polycystic kidney disease (PKD), and death 3 to 4 weeks after birth. Here, we examined the role of podocyte-derived laminin alpha5 via podocyte-specific inactivation of Lama5 and podocyte-specific rescue of the Lama5(neo) mutation. Podocyte-specific inactivation of Lama5 resulted in varying degrees of proteinuria and rates of progression to nephrotic syndrome. The GBM of proteinuric mice appeared thickened and "moth-eaten," and podocyte foot processes became effaced. Podocyte-specific restoration of laminin alpha5 production using two distinct strategies in Lama5(neo/neo) mice resulted in the resolution of proteinuria, hematuria, and PKD. These results suggest that the development of normal GBM structure and function requires podocyte-derived laminin alpha5 during and after glomerulogenesis and present a unique mechanism for the pathogenesis of PKD in these mice.

• Authors: Seth Goldberg, Tracy L Adair-Kirk, Robert M Senior, Jeffrey H Miner
• Journal: Journal of the American Society of Nephrology : JASN (J Am Soc Nephrol) Vol. 21 Issue 4 Pg. 579-86 (Apr 2010) ISSN: 1533-3450 [Electronic] United States
• PMID: 20150535 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Laminin
• laminin alpha5

Topics

• Animals
• Glomerular Basement Membrane (physiology)
• Laminin (genetics, physiology)
• Mice
• Mice, Transgenic
• Polycystic Kidney Diseases (etiology, genetics)