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Effects of a bupivacaine nerve block on the axonal transport of Tumor Necrosis Factor-alpha (TNF-alpha) in a rat model of carrageenan-induced inflammation.

Abstract
Many pro-inflammatory cytokines are involved in the process of inflammatory pain. Bi directional axonal transport of Tumor Necrosis Factor-alpha (TNF-alpha) occurs in case of neuropathic pain induced by nerve ligation. We used an in vivo preparation with injection of carrageenan and fluorescent TNF-alpha in the territory of the saphenous nerve of rats to study this transport. We have shown that retrograde transport of TNF-alpha occurs after an inflammatory insult caused by the injection of carrageenan. This transport was likely mediated by the TNF receptor 1. A nerve block with bupivacaine totally abolishes the expression of the receptor in the dorsal root ganglion and the retrograde transport of TNF-alpha. In addition, bupivacaine at low concentrations (1-10 microM) was able to stop the axonal transport ex vivo. Tetrodotoxin was less efficacious for inhibiting the TNF-alpha transport and the rise in receptor expression and for inhibiting the axonal transport ex vivo. This may partly explain the efficacy of nerve blocks with bupivacaine to decrease the neurogenic inflammation and in a lower extent the long-term inhibition of hyperalgesic phenomenon observed in animals and in humans.
AuthorsStéphane Deruddre, Evelyne Combettes, Jean-Pierre Estebe, Jacques Duranteau, Dan Benhamou, Hélène Beloeil, Jean-Xavier Mazoit
JournalBrain, behavior, and immunity (Brain Behav Immun) Vol. 24 Issue 4 Pg. 652-9 (May 2010) ISSN: 1090-2139 [Electronic] Netherlands
PMID20144702 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright 2010 Elsevier Inc. All rights reserved.
Chemical References
  • Anesthetics, Local
  • Polysaccharides
  • Receptors, Tumor Necrosis Factor, Type I
  • Tumor Necrosis Factor-alpha
  • Tetrodotoxin
  • Carrageenan
  • Bupivacaine
Topics
  • Anesthetics, Local (administration & dosage)
  • Animals
  • Axonal Transport (drug effects, immunology)
  • Bupivacaine (pharmacology)
  • Carrageenan
  • Disease Models, Animal
  • Edema (immunology)
  • Femoral Nerve (drug effects)
  • Fluorescence
  • Foot (pathology)
  • Ganglia, Spinal (immunology, metabolism)
  • Injections, Subcutaneous
  • Male
  • Nerve Block (methods)
  • Neurogenic Inflammation (drug therapy, immunology, metabolism)
  • Polymerase Chain Reaction
  • Polysaccharides
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Tumor Necrosis Factor, Type I (genetics, metabolism)
  • Tetrodotoxin (administration & dosage)
  • Tumor Necrosis Factor-alpha (administration & dosage, metabolism)

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