Abstract | OBJECTIVE: This study investigates the therapeutic potential of hyperbaric oxygen therapy (HBO) in reducing hypoxia and improving engraftment of intraportal islet transplants by promoting angiogenesis. METHODS: RESULTS: HBO therapy significantly reduced HIF-1α, TUNEL and VEGF expression in islets at POD 7. In the non-HBO transplants, liver enhancement on DCE MRI peaked at POD 7 consistent with less mature vasculature but this enhancement was suppressed at POD 7 in the HBO-treated group. The number of new peri-islet vessels at POD 7 was not significantly different between HBO and control groups. CONCLUSION: These results are consistent with a hyperbaric oxygen-mediated decrease in hypoxia that appeared to enhance vessel maturation in the critical days following intraportal islet transplantation.
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Authors | Naoaki Sakata, Nathaniel K Chan, Robert P Ostrowski, John Chrisler, Pete Hayes, Sonny Kim, Andre Obenaus, John H Zhang, Eba Hathout |
Journal | Pediatric diabetes
(Pediatr Diabetes)
Vol. 11
Issue 7
Pg. 471-8
(Nov 2010)
ISSN: 1399-5448 [Electronic] Denmark |
PMID | 20144181
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | © 2010 John Wiley & Sons A/S. |
Chemical References |
- Hypoxia-Inducible Factor 1, alpha Subunit
- Vascular Endothelial Growth Factor A
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Topics |
- Animals
- Diabetes Mellitus, Experimental
(therapy)
- Hyperbaric Oxygenation
- Hypoxia-Inducible Factor 1, alpha Subunit
(metabolism)
- In Situ Nick-End Labeling
- Islets of Langerhans
(blood supply, physiology)
- Islets of Langerhans Transplantation
(methods)
- Magnetic Resonance Imaging
- Mice
- Mice, Inbred BALB C
- Neovascularization, Physiologic
(drug effects)
- Vascular Endothelial Growth Factor A
(metabolism)
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