Differentiation of variola, monkeypox, and vaccinia antisera by radioimmunoassay.

Abstract

Poxvirus antisera adsorbed with "homologous" and "heterologous" poxvirus-infected chorioallantoic membranes (CAM) were differentiated by solid-phase radioimmunoassay (RIA). Mixtures of the antiserum dilutions and infected CAM were added directly (without centrifugation) to poxvirus-infected CAM antigens affixed to wells of microtitration plates. The affixed antigens combined with unadsorbed antibodies, and the cross-reactive antigen-antibody complexes were removed by washing. The results showed that adsorption of an antiserum with variola-, vaccinia-, or uninfected-CAM antigen and subsequent reaction of each in RIA with monkeypox- and uninfected-CAM antigens allowed the identification of antivariola, antivaccinia, or antimonkeypox sera.

• Authors: H D Hutchinson, D W Ziegler, D E Wells, J H Nakano
• Journal: Bulletin of the World Health Organization (Bull World Health Organ) Vol. 55 Issue 5 Pg. 613-23 ( 1977) ISSN: 0042-9686 [Print] Switzerland
• PMID: 201390 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Antigens, Viral
• Immune Sera

Topics

• Antigens, Viral
• Immune Sera (analysis)
• Monkeypox virus (immunology)
• Poxviridae (immunology)
• Radioimmunoassay
• Vaccinia virus (immunology)
• Variola virus (immunology)