Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells.

Sanguilutine and chelilutine, quaternary benzo[c]phenanthridine alkaloids, were studied for their antiproliferative activities with regard to their ability to induce oxidative stress. We observed potent antiproliferative activities for both alkaloids against three tumour (HeLa; HL-60; A-2780) and two normal (V-79; LEP) cell lines. Both alkaloids were efficient inductors of apoptosis. Statistical analysis revealed higher toxicity for sanguilutine compared to chelilutine and unequal sensitivity with regard to individual cell lines, although independent of the character of the cell line (i.e. tumour vs. normal). Dihydrofluorescein diacetate staining was used to measure intracellular ROS accumulation after treatment with sanguilutine, chelilutine, sanguinarine, and chelerythrine. In addition, anti-oxidative effects were studied. The effects of the alkaloids were compared with the effects of commonly used anti-oxidants, such as trolox, caffeine acid, and chlorogenic acid. None of the tested alkaloids (0.1 and 1 microg/ml) increased ROS production. Pre-incubation of sanguinarine and chelilutine (at all tested concentrations) and sanguilutine and chelerythrine (1 microg/ml) decreased oxidative stress caused by H(2)O(2). These findings indicate high antiproliferative and pro-apoptotic effects of sanguilutine and chelilutine that are not accompanied by oxidative stress induction, to the contrary, both alkaloids showed anti-oxidative effects.
AuthorsZ Slunská, E Gelnarová, J Hammerová, E Táborská, I Slaninová
JournalToxicology in vitro : an international journal published in association with BIBRA (Toxicol In Vitro) Vol. 24 Issue 3 Pg. 697-706 (Apr 2010) ISSN: 1879-3177 [Electronic] England
PMID20138138 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright (c) 2010 Elsevier Ltd. All rights reserved.
Chemical References
  • Annexin A5
  • Antineoplastic Agents
  • Benzophenanthridines
  • Coloring Agents
  • Oxidants
  • Reactive Oxygen Species
  • Tetrazolium Salts
  • Thiazoles
  • chelilutine
  • sanguilutine
  • thiazolyl blue
  • Propidium
  • Hydrogen Peroxide
  • Annexin A5
  • Antineoplastic Agents
  • Apoptosis (drug effects)
  • Benzophenanthridines (pharmacology)
  • Cell Cycle (drug effects)
  • Cell Line, Tumor
  • Cell Proliferation (drug effects)
  • Coloring Agents
  • DNA Fragmentation (drug effects)
  • Humans
  • Hydrogen Peroxide (antagonists & inhibitors, toxicity)
  • In Situ Nick-End Labeling
  • Oxidants (toxicity)
  • Oxidative Stress (drug effects)
  • Propidium
  • Reactive Oxygen Species (metabolism)
  • Tetrazolium Salts
  • Thiazoles

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: