Abstract | PURPOSE: MATERIALS AND METHODS: Diabetes was induced in six-week-old male Long-Evans rats by intraperitoneal injection of streptozotocin (STZ) (75 mg/kg). The rats were divided into four experimental groups: non-diabetic control rats, untreated diabetic rats, and diabetic rats treated with a low (4 mg/kg/day) or high (16 mg/kg/day) oral dose of fidarestat. After four weeks of treatment, accumulated leukocytes in the retina were counted in vivo by acridine orange digital fluorography. Intercellular adhesion molecule-1 (ICAM-1) and VEGF-164 mRNA levels in the retina were analyzed using the quantitative reverse transcription-polymerase chain reaction. ICAM-1 protein expression in the retina was investigated by immunohistochemistry. RESULTS:
Fidarestat treatment significantly decreased concentrations of sorbitol and fructose in the retinas of STZ-induced diabetic rats. Leukocyte accumulation in the retinas of fidarestat-treated rats was significantly less than in the untreated diabetic group (P < 0.01). Fidarestat treatment significantly reduced the expression ICAM-1 mRNA, but not VEGF-164 mRNA, in the retina of diabetic rats. Immunohistochemical study also revealed the suppressive effect of fidarestat on expression of ICAM-1. CONCLUSIONS:
|
Authors | Tomoaki Hattori, Akihisa Matsubara, Kaori Taniguchi, Yuichiro Ogura |
Journal | Current eye research
(Curr Eye Res)
Vol. 35
Issue 2
Pg. 146-54
(Feb 2010)
ISSN: 1460-2202 [Electronic] England |
PMID | 20136425
(Publication Type: Journal Article)
|
Chemical References |
- Blood Glucose
- Fluorescent Dyes
- Imidazolidines
- RNA, Messenger
- Vascular Endothelial Growth Factor A
- vascular endothelial growth factor A, rat
- Intercellular Adhesion Molecule-1
- Fructose
- Sorbitol
- fidarestat
- Aldehyde Reductase
- Acridine Orange
|
Topics |
- Acridine Orange
- Administration, Oral
- Aldehyde Reductase
(antagonists & inhibitors)
- Animals
- Blood Glucose
(analysis)
- Diabetes Mellitus, Experimental
(metabolism, prevention & control)
- Diabetic Retinopathy
(metabolism, prevention & control)
- Endothelium, Vascular
(metabolism)
- Fluorescent Antibody Technique, Indirect
- Fluorescent Dyes
- Fluorophotometry
- Fructose
(metabolism)
- Imidazolidines
(administration & dosage)
- Intercellular Adhesion Molecule-1
(genetics)
- Leukocytes
(metabolism)
- Male
- Microscopy, Fluorescence
- RNA, Messenger
(metabolism)
- Rats
- Rats, Long-Evans
- Retinal Vessels
(drug effects, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Sorbitol
(metabolism)
- Vascular Endothelial Growth Factor A
(genetics)
|