Abstract |
In this work, we report the effects of a Bowman-Birk protease inhibitor, the Black-Eyed Pea Trypsin/Chymotrypsin Inhibitor - BTCI, purified from Vigna unguiculata seeds, on the MCF-7 breast cancer cells. The treatment of MCF-7 with 200microM BTCI for 72h induced significant reduction of the cell viability and proliferation (arrest in S and G2/M phase). These cytostatic effects were accompanied by acute morphological modifications including the alteration of the nuclear morphology, plasma membrane fragmentation, cytoplasm disorganization, presence of double-membrane vesicles, mitochondrial swelling, and an increase in the size of lysosomes. Significative DNA fragmentation, annexin-V(+) cell number increase, mitochondrial membrane potential reduction, and cytoplasm acidification were also detected. All together, these cytostatic and cytotoxic results point out to BTCI-induced apoptosis cell death associated with severe cell morphological alterations and lysosome membrane permeabilization. Our study confirms the anticarcinogenic potential of Bowman-Birk protease inhibitors and identifies BTCI as a promising tool for drug developments aimed at the treatment of breast cancer.
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Authors | Graziella Anselmo Joanitti, Ricardo Bentes Azevedo, Sonia Maria Freitas |
Journal | Cancer letters
(Cancer Lett)
Vol. 293
Issue 1
Pg. 73-81
(Jul 01 2010)
ISSN: 1872-7980 [Electronic] Ireland |
PMID | 20133052
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Plant Proteins
- Trypsin Inhibitors
- trypsin, chymotrypsin inhibitor, black-eyed pea
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Topics |
- Apoptosis
(drug effects)
- Breast Neoplasms
(drug therapy, metabolism, pathology)
- Cell Cycle
(drug effects)
- Cell Growth Processes
(drug effects)
- Cell Line, Tumor
- Cell Membrane Permeability
(drug effects)
- Cell Survival
(drug effects)
- Fabaceae
(chemistry)
- Female
- Humans
- Lysosomes
(drug effects, metabolism)
- Membrane Potential, Mitochondrial
(drug effects)
- Microscopy, Fluorescence
- Plant Proteins
(isolation & purification, pharmacology)
- Seeds
(chemistry)
- Trypsin Inhibitors
(isolation & purification, pharmacology)
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