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Apoptosis and lysosome membrane permeabilization induction on breast cancer cells by an anticarcinogenic Bowman-Birk protease inhibitor from Vigna unguiculata seeds.

Abstract
In this work, we report the effects of a Bowman-Birk protease inhibitor, the Black-Eyed Pea Trypsin/Chymotrypsin Inhibitor - BTCI, purified from Vigna unguiculata seeds, on the MCF-7 breast cancer cells. The treatment of MCF-7 with 200microM BTCI for 72h induced significant reduction of the cell viability and proliferation (arrest in S and G2/M phase). These cytostatic effects were accompanied by acute morphological modifications including the alteration of the nuclear morphology, plasma membrane fragmentation, cytoplasm disorganization, presence of double-membrane vesicles, mitochondrial swelling, and an increase in the size of lysosomes. Significative DNA fragmentation, annexin-V(+) cell number increase, mitochondrial membrane potential reduction, and cytoplasm acidification were also detected. All together, these cytostatic and cytotoxic results point out to BTCI-induced apoptosis cell death associated with severe cell morphological alterations and lysosome membrane permeabilization. Our study confirms the anticarcinogenic potential of Bowman-Birk protease inhibitors and identifies BTCI as a promising tool for drug developments aimed at the treatment of breast cancer.
AuthorsGraziella Anselmo Joanitti, Ricardo Bentes Azevedo, Sonia Maria Freitas
JournalCancer letters (Cancer Lett) Vol. 293 Issue 1 Pg. 73-81 (Jul 01 2010) ISSN: 1872-7980 [Electronic] Ireland
PMID20133052 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Plant Proteins
  • Trypsin Inhibitors
  • trypsin, chymotrypsin inhibitor, black-eyed pea
Topics
  • Apoptosis (drug effects)
  • Breast Neoplasms (drug therapy, metabolism, pathology)
  • Cell Cycle (drug effects)
  • Cell Growth Processes (drug effects)
  • Cell Line, Tumor
  • Cell Membrane Permeability (drug effects)
  • Cell Survival (drug effects)
  • Fabaceae (chemistry)
  • Female
  • Humans
  • Lysosomes (drug effects, metabolism)
  • Membrane Potential, Mitochondrial (drug effects)
  • Microscopy, Fluorescence
  • Plant Proteins (isolation & purification, pharmacology)
  • Seeds (chemistry)
  • Trypsin Inhibitors (isolation & purification, pharmacology)

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