Abstract | PURPOSE: To determine the influence of low-power laser (660 nm) on the collagen percentage and macrophages in skin wounds in diabetic rats. METHODS: 30 male Wistar rats were used, distributed in two groups: laser treated diabetic (n= 15); untreated diabetic (n = 15). The diabetes was induced by intravenous injection of alloxan into the dorsal vein of the penis, at a rate of 0.1 ml of solution per 100 g of body weight. A wound was made on the back of all the animals. Groups 2 were treated with Aluminium Gallium Indium Phosphide - InGaAlP type diode laser (Photon Laser III DMC) with a continuous output power of 100 mW and wavelength (lambda) of 660 nm (4 J/cm(2)) for 24 s. five animal from each group was sacrificed on the 3rd, 7th and 14th days after wounding. Samples were taken, embedded in paraffin, stained with hematoxylin- eosin, Masson's trichrome, and immunohistochemical macrophage. morphometrically analyzed using the Image Pro Plus 4.5 software. The percentages of collagen fibers and macrophages were determined from the samples from the euthanasia animals. RESULTS: The data were treated statistically using analysis of variance (ANOVA) and the Post-hocTukey test. The significance level was set at 0.05 or 5%. CONCLUSION: The low-power laser (660 nm) was shown to be capable of influencing the collagen percentage in skin wounds by increasing the mean quantity of collagen fibers and macrophages.
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Authors | Paulo de Tarso Camillo de Carvalho, Iandara Schettert da Silva, Filipe Abdalla dos Reis, Daniel Martins Perreira, Ricardo Dutra Aydos |
Journal | Acta cirurgica brasileira
(Acta Cir Bras)
Vol. 25
Issue 1
Pg. 71-9
(Feb 2010)
ISSN: 1678-2674 [Electronic] Brazil |
PMID | 20126892
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Analysis of Variance
- Animals
- Collagen
(biosynthesis, radiation effects)
- Diabetes Mellitus, Experimental
(chemically induced)
- Disease Models, Animal
- Low-Level Light Therapy
(methods)
- Macrophages
(radiation effects)
- Male
- Random Allocation
- Rats
- Rats, Wistar
- Skin
(injuries)
- Wound Healing
(radiation effects)
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