Abstract | OBJECTIVE: STUDY DESIGN: Seventy single-rooted maxillary central teeth were used in this study. All teeth were instrumented to size 50 using a step-back technique. The selected teeth were randomly divided into 4 groups, each containing 15 experimental samples and 5 positive and 5 negative control samples. In the first and second groups (smear+), the teeth were irrigated with only 5.25% NaOCl. In the third and fourth groups (smear-), the teeth were irrigated with 17% EDTA and 5.25% NaOCl to remove the smear layer. Also, in the first and third groups, cavities were prepared as 3 mm. In the second and fourth groups, cavities were prepared as 5 mm. All the root-end cavities were then filled with MTA. Nail varnish was applied to all external root surfaces to the level of the resected root-ends to prevent lateral microleakage. Samples were sterilized in an ethylene oxide sterilizer for 12 hours. The apical 3-4 mm of the roots were immersed in brain heart infusion culture medium with phenol red indicator within culture chambers. The coronal access of each specimen was inoculated every 48 hours with a suspension of Enterococcus faecalis. Bacterial leakage was monitored every 24 hours for 4 weeks. The data obtained were analyzed using a chi-squared test, with alpha = .05 as the level for statistical significance. RESULTS: There were no statistically significant differences in rate of bacterial leakage among the experimental groups at 1-4 weeks (P > .05). Also, there was no difference between the groups when the 2 thickness groups were combined (P > .05). However, there was statistically significant differences when the 2 smear groups were combined for 4-week observation periods (P < .05). Removal of the smear layer caused significantly more apical microleakage than when the smear layer was left intact for 4 weeks. CONCLUSION: The thickness of root-end cavity (3 or 5 mm) had no influence in the bacterial leakage of the root end filled with MTA. Removing the smear layer may not be necessary in root-end cavities filled with MTA.
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Authors | Tahsin Yildirim, Kürşat Er, Tamer Taşdemir, Erhan Tahan, Kurtuluş Buruk, Ahmet Serper |
Journal | Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics
(Oral Surg Oral Med Oral Pathol Oral Radiol Endod)
Vol. 109
Issue 1
Pg. e67-72
(Jan 2010)
ISSN: 1528-395X [Electronic] United States |
PMID | 20123373
(Publication Type: Journal Article)
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Copyright | Copyright 2010 Mosby, Inc. All rights reserved. |
Chemical References |
- Aluminum Compounds
- Calcium Compounds
- Drug Combinations
- Oxides
- Root Canal Filling Materials
- Silicates
- mineral trioxide aggregate
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Topics |
- Aluminum Compounds
- Calcium Compounds
- Chi-Square Distribution
- Dental Leakage
(prevention & control)
- Dental Pulp Cavity
(microbiology, pathology)
- Drug Combinations
- Enterococcus faecalis
- Humans
- Incisor
- Maxilla
- Oxides
- Retrograde Obturation
(instrumentation, methods)
- Root Canal Filling Materials
- Silicates
- Smear Layer
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