Cardioprotection afforded by St Thomas solution is enhanced by emulsified isoflurane in an isolated heart ischemia reperfusion injury model in rats.

Abstract

OBJECTIVE:

The purpose of this study was to investigate whether adding emulsified isoflurane to St Thomas cardioplegia solution could enhance the cardiac protection after cardioplegic arrest in rats.

DESIGN:

A randomized, blind study.

SETTING:

A university laboratory.

PARTICIPANTS:

Thirty male Sprague-Dawley rats.

INTERVENTIONS:

Thirty isolated heart preparations were randomly divided into 3 groups (n = 10/group) according to the different cardioplegia solutions being given: St Thomas solution mixed with emulsified isoflurane (containing 2.8% of isoflurane, group EI), St Thomas solution mixed with emulsified Intralipid (Huarui Pharmacy, Wuxi, Jiangsu, China) (group EL), and St Thomas solution alone (group St). In the 35-minute normothermic ischemia period, infusion of cardioplegia solution was repeated every 15 minutes. After the 35-minute ischemia period, the heart was perfused with Krebs-Henseleit buffer for another 2 hours.

MEASUREMENTS AND MAIN RESULTS:

The functional parameters of the heart were monitored throughout the experiments. The coronary effluent was collected for measuring the activity of CK-MB 30 minutes after reperfusion, and the infarct size was assessed at the end of reperfusion. The infarct size in group EI (24% +/- 4%) was reduced when compared with that in group EL (31% +/- 8%, p < 0.05) and group St (43% +/- 9%, p < 0.001). The CK-MB activity in group EI was decreased significantly when compared with that in group EL and group St (p < 0.05). The functional recovery in group EI also was improved. Compared with standard St Thomas solution alone, adding 30% Intralipid alone also significantly reduced the infarct size and the CK-MB leakage and improved the recovery of the mechanical function.

CONCLUSIONS:

St Thomas cardioplegia solution supplemented with emulsified isoflurane enhanced its cardioprotection in an isolated heart ischemia reperfusion injury model in rats.

• Authors: Han Huang, Wensheng Zhang, Shanling Liu, Chen Yanfang, Tao Li, Jin Liu
• Journal: Journal of cardiothoracic and vascular anesthesia (J Cardiothorac Vasc Anesth) Vol. 24 Issue 1 Pg. 99-103 (Feb 2010) ISSN: 1532-8422 [Electronic] United States
• PMID: 20123239 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2010 Elsevier Inc. All rights reserved.

Chemical References

• Anesthetics, Inhalation
• Bicarbonates
• Cardioplegic Solutions
• Fat Emulsions, Intravenous
• St. Thomas' Hospital cardioplegic solution
• Sodium Chloride
• Potassium Chloride
• Isoflurane
• Creatine Kinase, MB Form
• Magnesium
• Calcium Chloride

Topics

• Anesthetics, Inhalation (pharmacology)
• Animals
• Bicarbonates (chemistry, pharmacology)
• Calcium Chloride (chemistry, pharmacology)
• Cardioplegic Solutions (pharmacology)
• Creatine Kinase, MB Form (analysis)
• Disease Models, Animal
• Fat Emulsions, Intravenous (pharmacology)
• Heart (drug effects)
• Hemodynamics (drug effects)
• Isoflurane (pharmacology)
• Magnesium (chemistry, pharmacology)
• Male
• Myocardial Infarction (pathology, prevention & control)
• Potassium Chloride (chemistry, pharmacology)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (drug therapy)
• Sodium Chloride (chemistry, pharmacology)
• Therapeutics