Restoration of peripheral blood T cell repertoire complexity during remission in advanced cutaneous T cell lymphoma.

Abstract	In advanced stages, cutaneous T cell lymphomas (CTCL) are associated with increased mortality from infections and also increased susceptibility to skin malignancies. In this study, we analyzed the complexity of the peripheral blood T cell repertoire with a sensitive b-variable (BV) complementarity-determining region 3 (CDR3) spectratyping analysis and flow cytometry in three-stage IV CTCL/Sezary syndrome patients who achieved complete clinical remission after therapy. The T cell repertoire of peripheral blood T cells before treatment was profoundly abnormal across multiple BV subfamilies. Following treatment, CDR3 spectratype patterns showed dramatic restoration of normal diversity and complexity. However, absolute CD4 counts across multiple BV families remained low for many months, even after identifiable circulating malignant T cell populations were eliminated. These data suggest that the diversity of the T cell repertoire can be recovered after successful treatment of even advanced CTCL.
Authors	Kei-ichi Yamanaka, Robert C Fuhlbrigge, Hitoshi Mizutani, Thomas S Kupper
Journal	Archives of dermatological research (Arch Dermatol Res) Vol. 302 Issue 6 Pg. 453-9 (Aug 2010) ISSN: 1432-069X [Electronic] Germany
PMID	20111968 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
Chemical References	Diphtheria Toxin Interferon-alpha Interleukin-2 Receptors, Antigen, T-Cell Recombinant Fusion Proteins denileukin diftitox
Topics	Aged Aged, 80 and over Blood Circulation (immunology) CD4-Positive T-Lymphocytes (drug effects, immunology, metabolism, pathology) Cell Separation Diphtheria Toxin (therapeutic use) Female Flow Cytometry Humans Interferon-alpha (therapeutic use) Interleukin-2 (therapeutic use) Lymphoma, T-Cell, Cutaneous (drug therapy, genetics, pathology, physiopathology) Male Middle Aged Neoplasm Staging Receptors, Antigen, T-Cell (genetics) Recombinant Fusion Proteins (therapeutic use) Remission Induction Skin (blood supply, immunology, pathology) T-Lymphocyte Subsets (drug effects, immunology, metabolism, pathology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!