Abstract | BACKGROUND/AIM: METHODS:
Bilitranslocase expression was evaluated in renal homogenates (H), renal basolateral plasma membranes (KBLM) and liver plasma membranes (LPM) by immunoblotting. Bilitranslocase function was studied by measuring the kinetic parameters of electrogenic bromosulfophthalein (BSP) uptake in KBLM and LPM by a spectrophotometric technique. RESULTS: An increased abundance of bilitranslocase in KBLM without modifications in renal H and in LPM from BDL rats was observed compared with Sham rats. BDL rats showed a higher V(max) for BSP uptake in KBLM. No differences between groups were observed for Michaelis-Menten parameters in LPM. CONCLUSION: The higher renal expression and function of bilitranslocase in renal basolateral membranes from rats with obstructive cholestasis might also contribute to the dramatic increase in BSP renal excretion observed in this experimental model. This would be another compensation mechanism to overcome the hepatic dysfunction in the elimination of organic anions.
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Authors | Anabel Brandoni, Gisela Di Giusto, Raffaella Franca, Sabina Passamonti, Adriana M Torres |
Journal | Nephron. Physiology
(Nephron Physiol)
Vol. 114
Issue 4
Pg. p35-40
( 2010)
ISSN: 1660-2137 [Electronic] Switzerland |
PMID | 20110735
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2010 S. Karger AG, Basel. |
Chemical References |
- Membrane Proteins
- Ceruloplasmin
- bilitranslocase
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Topics |
- Acute Disease
- Animals
- Biological Transport, Active
(physiology)
- Ceruloplasmin
- Cholestasis
(enzymology)
- Gene Expression Regulation, Enzymologic
- Kidney
(enzymology, pathology)
- Liver
(enzymology, pathology)
- Male
- Membrane Proteins
(biosynthesis, genetics)
- Rats
- Rats, Wistar
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