Abstract | PURPOSE: METHODS: Female Sprague-Dawley rats were divided into 3 groups: control (n = 5), chronic liver injury (alpha-naphtylisocyocyanate [ANIT] every 3.5 days at 75 mg/kg; n = 5), and chronic liver injury plus HGF (ANIT + HGF at 250 microg kg(-1) d(-1); n = 5). The rats initially underwent massive (80%) small bowel resections. Seven days later, they were given intraperitoneal injections of saline (control) or ANIT and implantation of an osmotic minipump for continuous intravenous saline or HGF. Intraperitoneal saline or ANIT injections were subsequently administered every 3.5 days to create a chronic cholestatic model. After 14 days, the animals were euthanized, and liver biopsies were obtained. The liver biopsies were evaluated by histology, immunofluorescence staining for interleukin-6 and tumor necrosis factor alpha, and assessment of apoptosis by terminal dUTP- transferase-mediated nick end labeling (TUNEL) technique. RESULTS: CONCLUSION:
Hepatocyte growth factor preserved the hepatic ductal system, modulated the hepatic inflammatory response, and reduced the apoptotic index in this chronic cholestatic liver injury model. It may diminish or prevent liver damage in patients with total parenteral nutrition-induced liver injury.
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Authors | Keith A Thatch, Michael S Katz, Marian M Haber, Marshall Z Schwartz |
Journal | Journal of pediatric surgery
(J Pediatr Surg)
Vol. 45
Issue 1
Pg. 89-94
(Jan 2010)
ISSN: 1531-5037 [Electronic] United States |
PMID | 20105586
(Publication Type: Journal Article)
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Copyright | Copyright 2010 Elsevier Inc. All rights reserved. |
Chemical References |
- Interleukin-6
- Tumor Necrosis Factor-alpha
- 1-Naphthylisothiocyanate
- Hepatocyte Growth Factor
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Topics |
- 1-Naphthylisothiocyanate
- Animals
- Chemical and Drug Induced Liver Injury
(therapy)
- Cholestasis, Intrahepatic
(etiology, prevention & control, therapy)
- Disease Models, Animal
- Female
- Hepatocyte Growth Factor
(pharmacology, therapeutic use)
- Immunohistochemistry
- In Situ Nick-End Labeling
- Interleukin-6
(analysis, metabolism)
- Liver
(chemistry, drug effects, pathology)
- Liver Diseases
(drug therapy, etiology, pathology)
- Liver Failure
(prevention & control)
- Liver Function Tests
- Parenteral Nutrition, Total
(adverse effects)
- Rats
- Rats, Sprague-Dawley
- Short Bowel Syndrome
(therapy)
- Tumor Necrosis Factor-alpha
(analysis)
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