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[Fluorescent microbeads-based multiplex detection of IgM antibodies to pathogens caused viral hemorrhagic fever].

AbstractOBJECTIVE:
To develop and evaluate a multiplex detection of IgM antibodies to pathogens caused viral hemorrhagic fever.
METHODS:
The nucleocapsid proteins (NP) of HTN, SEO, Puu MBV, Lassa, RFV and HPS viruses expressed in prokaryotic cells and purified were coupled to 7 different xMAP fluorescent microbeads. The assay was evaluated and optimized when screened against a panel of reference sera collected from HFRS patients, and compared to commonly used MacELISA Kits.
RESULTS:
For detection of anti-NP antibodies, the sensitivity and specificity of the assay were comparable to a commonly used MacELISA kit, but it could detect different antigen specific antibodies in one reaction simultaneously.
CONCLUSION:
A robust, rapid and multiplex assay based on NPs could be developed via Luminex xMAP platform for laboratory diagnosis of viral hemorrhagic fever and seroepidemiological investigation.
AuthorsJian-dong Li, Shuo Zhang, Quan-fu Zhang, Qin-zhi Liu, Yan Wei, Chuan Li, Mi-fang Liang, De-xin Li
JournalZhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology (Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi) Vol. 23 Issue 2 Pg. 149-51 (Apr 2009) ISSN: 1003-9279 [Print] China
PMID20104764 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Viral
  • Immunoglobulin M
  • Nucleocapsid Proteins
Topics
  • Antibodies, Viral (blood)
  • Fluorescence
  • Hemorrhagic Fevers, Viral (blood, immunology, virology)
  • Humans
  • Immunoassay (methods)
  • Immunoglobulin M (blood)
  • Microspheres
  • Nucleocapsid Proteins (chemistry, immunology)
  • Viruses (immunology)

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