Abstract | OBJECTIVE: METHODS: The nucleocapsid proteins (NP) of HTN, SEO, Puu MBV, Lassa, RFV and HPS viruses expressed in prokaryotic cells and purified were coupled to 7 different xMAP fluorescent microbeads. The assay was evaluated and optimized when screened against a panel of reference sera collected from HFRS patients, and compared to commonly used MacELISA Kits. RESULTS: For detection of anti-NP antibodies, the sensitivity and specificity of the assay were comparable to a commonly used MacELISA kit, but it could detect different antigen specific antibodies in one reaction simultaneously. CONCLUSION: A robust, rapid and multiplex assay based on NPs could be developed via Luminex xMAP platform for laboratory diagnosis of viral hemorrhagic fever and seroepidemiological investigation.
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Authors | Jian-dong Li, Shuo Zhang, Quan-fu Zhang, Qin-zhi Liu, Yan Wei, Chuan Li, Mi-fang Liang, De-xin Li |
Journal | Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology
(Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi)
Vol. 23
Issue 2
Pg. 149-51
(Apr 2009)
ISSN: 1003-9279 [Print] China |
PMID | 20104764
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Viral
- Immunoglobulin M
- Nucleocapsid Proteins
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Topics |
- Antibodies, Viral
(blood)
- Fluorescence
- Hemorrhagic Fevers, Viral
(blood, immunology, virology)
- Humans
- Immunoassay
(methods)
- Immunoglobulin M
(blood)
- Microspheres
- Nucleocapsid Proteins
(chemistry, immunology)
- Viruses
(immunology)
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