Abstract |
Polyaniline/ carbon nanotubes composite (PANI-CNT) electrochemically deposited onto indium-tin-oxide (ITO) coated glass plate has been utilized for Neisseria gonorrhoeae detection by immobilizing 5'-amino-labeled Neisseria gonorrhoeae probe (aDNA) using glutaraldehyde as a cross-linker. PANI-CNT/ITO and aDNA-Glu-PANI-CNT/ITO electrodes have been characterized using scanning electron microscopy (SEM), Fourier Transform Infrared (FT-IR) spectroscopy, cyclic voltammetry (CV), and differential pulse voltammetry (DPV). This bioelectrode can be used to detect N. gonorrhoeae using methylene blue (MB) as redox indicator with response time of 60 s and stability of about 75 days when stored under refrigerated conditions. DPV studies reveal that this bioelectrode can detect complementary DNA concentration from 1 x 10(-6) M to 1 x 10(-17) M with detection limit of 1.2 x 10(-17) M. Further, this bioelectrode (aDNA-Glu-PANI-CNT/ITO) exhibits specificity toward N. gonorrhoeae species and shows negative response with non-Neisseria gonorrhoeae Neisseria species (NgNS) and other gram negative bacteria (GNB).
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Authors | Renu Singh, Chetna Dhand, Gajjala Sumana, Rachna Verma, Seema Sood, Rajinder Kumar Gupta, Bansi Dhar Malhotra |
Journal | Journal of molecular recognition : JMR
(J Mol Recognit)
2010 Sep-Oct
Vol. 23
Issue 5
Pg. 472-9
ISSN: 1099-1352 [Electronic] England |
PMID | 20101643
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Aniline Compounds
- DNA, Bacterial
- Nanotubes, Carbon
- polyaniline
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Topics |
- Aniline Compounds
(chemistry)
- Biosensing Techniques
(instrumentation, methods)
- DNA, Bacterial
(chemistry)
- Electrochemistry
(instrumentation, methods)
- Gonorrhea
(diagnosis)
- Humans
- Limit of Detection
- Nanotubes, Carbon
(chemistry)
- Neisseria gonorrhoeae
(chemistry, genetics)
- Sensitivity and Specificity
- Sexually Transmitted Diseases
(diagnosis)
- Spectroscopy, Fourier Transform Infrared
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