HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Polyaniline/carbon nanotubes platform for sexually transmitted disease detection.

Abstract
Polyaniline/carbon nanotubes composite (PANI-CNT) electrochemically deposited onto indium-tin-oxide (ITO) coated glass plate has been utilized for Neisseria gonorrhoeae detection by immobilizing 5'-amino-labeled Neisseria gonorrhoeae probe (aDNA) using glutaraldehyde as a cross-linker. PANI-CNT/ITO and aDNA-Glu-PANI-CNT/ITO electrodes have been characterized using scanning electron microscopy (SEM), Fourier Transform Infrared (FT-IR) spectroscopy, cyclic voltammetry (CV), and differential pulse voltammetry (DPV). This bioelectrode can be used to detect N. gonorrhoeae using methylene blue (MB) as redox indicator with response time of 60 s and stability of about 75 days when stored under refrigerated conditions. DPV studies reveal that this bioelectrode can detect complementary DNA concentration from 1 x 10(-6) M to 1 x 10(-17) M with detection limit of 1.2 x 10(-17) M. Further, this bioelectrode (aDNA-Glu-PANI-CNT/ITO) exhibits specificity toward N. gonorrhoeae species and shows negative response with non-Neisseria gonorrhoeae Neisseria species (NgNS) and other gram negative bacteria (GNB).
AuthorsRenu Singh, Chetna Dhand, Gajjala Sumana, Rachna Verma, Seema Sood, Rajinder Kumar Gupta, Bansi Dhar Malhotra
JournalJournal of molecular recognition : JMR (J Mol Recognit) 2010 Sep-Oct Vol. 23 Issue 5 Pg. 472-9 ISSN: 1099-1352 [Electronic] England
PMID20101643 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Aniline Compounds
  • DNA, Bacterial
  • Nanotubes, Carbon
  • polyaniline
Topics
  • Aniline Compounds (chemistry)
  • Biosensing Techniques (instrumentation, methods)
  • DNA, Bacterial (chemistry)
  • Electrochemistry (instrumentation, methods)
  • Gonorrhea (diagnosis)
  • Humans
  • Limit of Detection
  • Nanotubes, Carbon (chemistry)
  • Neisseria gonorrhoeae (chemistry, genetics)
  • Sensitivity and Specificity
  • Sexually Transmitted Diseases (diagnosis)
  • Spectroscopy, Fourier Transform Infrared

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: