Abstract |
The effect of o- phthalic acid (o-PA) on the production of the anti- tumor polyketide compound aspergiolide A by the marine fungus Aspergillus glaucus was investigated. o-PA at 12mM increased the aspergiolide A production by 77%. A combination of 12mM acetate with 12mM o-PA increased the production by 126%, i.e., from 27.1mg/l to 60.9mg/l, which was 27% and 75% higher than adding either 12mM o-PA or 12mM acetate, respectively. It was also found that A. glaucus could use o-PA as the sole carbon source on Minimal Medium. Ninety percent of o-PA was degraded by the fungus strain, especially when 12mM acetate was supplemented simultaneously. In culture medium supplemented with o-PA addition, sugar consumption decreased with increasing o-PA concentration, at the same time, the oxalacetate and pyruvate levels increased and the concentration of fumarate decreased. The results indicated that o-PA could stimulate biosynthesis of aspergiolide A effectively by regulating the metabolic pathway.
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Authors | Xueqian Sun, Xiangshan Zhou, Menghao Cai, Jiushun Zhou, Yuanxing Zhang |
Journal | Bioresource technology
(Bioresour Technol)
Vol. 101
Issue 10
Pg. 3609-16
(May 2010)
ISSN: 1873-2976 [Electronic] England |
PMID | 20100655
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2010 Elsevier Ltd. All rights reserved. |
Chemical References |
- Anthraquinones
- Culture Media
- Phthalic Acids
- aspergiolide A
- phthalic acid
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Topics |
- Anthraquinones
(metabolism)
- Aspergillus
(drug effects, metabolism)
- Biomass
- Chromatography, High Pressure Liquid
- Culture Media
- Phthalic Acids
(pharmacology)
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