Abstract | INTRODUCTION: METHODS: Serum samples of 134 patients with untreated mycobacterium infections (122 TB, 12 nontuberculous mycobacterium) were tested for antibodies against both the citrullinated ( Cit) and the non-citrullinated (Arg) form of 2 cyclic synthetic peptides. In 33 patients, a follow-up sample was tested six months after starting anti-mycobacterial drugs. RESULTS: A substantial proportion of patients with mycobacterial infections demonstrated antibodies against 0401Cit, 0401Arg, 0722Cit and 0722Arg. Fourteen patients demonstrated anti-0401Cit, 83 anti-0401Arg, 22 anti-0722Cit and 61 anti-0722Arg, while none of these antibodies were detected in the 20 healthy controls. All the patients but one, who were anti-0401Cit and anti-0722Cit positive, demonstrated reactivity against the respective Arg peptide. In the subset of 33 patients with a follow-up test six months after starting treatment, the mean levels of antibodies to 0401Cit, 0401Arg, 0722Cit and 0722Arg significantly decreased after treatment. All the patients who were anti-0401Cit and anti-0722Cit positive turned negative after treatment. The presence of anti-0401Cit/Arg and anti-0722Cit/Arg was found to be significantly correlated with the presence of HIV. CONCLUSIONS: ACPA may be found in patients with TB. In most of the cases, the reactivity is citrulline independent. A positive cyclic citrullinated peptide (CCP) test in these patients should therefore be interpreted with care, and preferably followed by a control ELISA with a non-citrullinated antigen.
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Authors | Ori Elkayam, Refael Segal, Daniele Bendayan, Robert van Uitert, Carla Onnekink, Ger Jm Pruijn |
Journal | Arthritis research & therapy
(Arthritis Res Ther)
Vol. 12
Issue 1
Pg. R12
( 2010)
ISSN: 1478-6362 [Electronic] England |
PMID | 20100318
(Publication Type: Journal Article)
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Chemical References |
- Antitubercular Agents
- Autoantibodies
- Peptides, Cyclic
- Proteins
- cyclic citrullinated peptide
- Citrulline
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Topics |
- Adolescent
- Adult
- Aged
- Aged, 80 and over
- Antitubercular Agents
(therapeutic use)
- Autoantibodies
(blood)
- Citrulline
(metabolism)
- Enzyme-Linked Immunosorbent Assay
- Female
- HIV Infections
(blood, complications, immunology)
- Humans
- Male
- Middle Aged
- Peptides, Cyclic
(immunology)
- Proteins
(immunology, metabolism)
- Tuberculosis
(blood, complications, immunology)
- Young Adult
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