Abstract |
The present study aimed at understanding the effect of the linoleic acid derivative 8-[2-(2-pentyl-cyclopropylmethyl)-cyclopropyl]- octanoic acid ( DCP-LA) on oxidative stress-induced neuronal death. Sodium nitroprusside (SNP; 1 mM) reduced viability of cultured rat cerebral cortical neurons to 50% of basal levels, but DCP-LA significantly prevented the SNP effect in a concentration (1-100 nM)-dependent manner. In addition, DCP-LA (100 nM) rescued neurons from SNP-induced degradation. SNP (1 mM) activated caspase-3 and -9 in cultured rat cerebral cortical neurons, but DCP-LA (100 nM) abolished the caspase activation. For a mouse model of middle cerebral artery occlusion, oral administration with DCP-LA (1 mg/kg) significantly diminished degraded area due to cerebral infarction. The results of the present study, thus, demonstrate that DCP-LA protects neurons at least in part from oxidative stress-induced apoptosis by inhibiting activation of caspase-3/-9.
|
Authors | Takahiro Yaguchi, Hirokazu Fujikawa, Tomoyuki Nishizaki |
Journal | Neurochemical research
(Neurochem Res)
Vol. 35
Issue 5
Pg. 712-7
(May 2010)
ISSN: 1573-6903 [Electronic] United States |
PMID | 20099079
(Publication Type: Journal Article)
|
Chemical References |
- 8-(2-(2-pentyl-cyclopropylmethyl)cyclopropyl)octanoic acid
- Caprylates
- Nitroprusside
- Caspase 3
- Caspase 9
|
Topics |
- Animals
- Apoptosis
(drug effects)
- Brain Ischemia
(prevention & control)
- Caprylates
(pharmacology)
- Caspase 3
(metabolism)
- Caspase 9
(metabolism)
- Cells, Cultured
- Enzyme Activation
(drug effects)
- Infarction, Middle Cerebral Artery
(drug therapy)
- Male
- Mice
- Neurons
(drug effects, pathology)
- Nitroprusside
(pharmacology)
- Oxidative Stress
(drug effects)
- Rats
|