Abstract | BACKGROUND: RESULTS: Here we show that IL1RAPL1 is present in dendritic spine where it interacts with PSD-95, a major component of excitatory postsynaptic compartment. Using gain- and loss-of-function experiments in neurons, we demonstrated that IL1RAPL1 regulates the synaptic localization of PSD-95 by controlling c-Jun terminal kinase (JNK) activity and PSD-95 phosphorylation. Mice carrying a null mutation of the mouse Il1rapl1 gene show a reduction of both dendritic spine density and excitatory synapses in the CA1 region of the hippocampus. These structural abnormalities are associated with specific deficits in hippocampal long-term synaptic plasticity. CONCLUSION: The interaction of IL1RAPL1 with PSD-95 discloses a novel pathophysiological mechanism of cognitive impairment associated with alterations of the JNK pathway leading to a mislocalization of PSD-95 and abnormal synaptic organization and function.
|
Authors | Alice Pavlowsky, Antonella Gianfelice, Marta Pallotto, Alice Zanchi, Hugo Vara, Malik Khelfaoui, Pamela Valnegri, Xavier Rezai, Silvia Bassani, Dario Brambilla, Jiri Kumpost, Jaroslav Blahos, Michel J Roux, Yann Humeau, Jamel Chelly, Maria Passafaro, Maurizio Giustetto, Pierre Billuart, Carlo Sala |
Journal | Current biology : CB
(Curr Biol)
Vol. 20
Issue 2
Pg. 103-15
(Jan 26 2010)
ISSN: 1879-0445 [Electronic] England |
PMID | 20096586
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright 2010 Elsevier Ltd. All rights reserved. |
Chemical References |
- Disks Large Homolog 4 Protein
- Dlg4 protein, rat
- IL1RAPL1 protein, rat
- Interleukin-1 Receptor Accessory Protein
- Intracellular Signaling Peptides and Proteins
- Membrane Proteins
|
Topics |
- Animals
- Cognition
- Disks Large Homolog 4 Protein
- Hippocampus
(cytology, metabolism)
- Interleukin-1 Receptor Accessory Protein
(genetics, physiology)
- Intracellular Signaling Peptides and Proteins
(metabolism)
- Membrane Proteins
(metabolism)
- Mice
- Mutation
- PC12 Cells
- Phosphorylation
- Rats
- Signal Transduction
- Synapses
(metabolism)
|