144 SD rats were randomly divided into four experimental groups: control group (6 rats),
PDTC group (36 rats), PQ group (56 rats) and PQ +
PDTC group (46 rats). On the 1st, the 3rd, the 7th, the 14th, the 28th and the 56th day
after treatment, the level of
malondialdehyde (MDA), the activity of
glutathione peroxidase (GSH-Px),
superoxide dismutase (SOD),
catalase (CAT) and
myeloperoxidase (MPO) in serum were detected; the content of
hydroxyproline (Hyp) and the activity of
NF-kappaB in lung tissues were detected; the lung pathological changes of rats were observed.
RESULTS: The level of MDA and MPO in serum increased and the activity of GSH-Px, SOD, CAT in serum decreased significantly in PQ group compared with control and
PDTC group (P<0.05 or P<0.01) in the corresponding sacrifice dates. There were a significant decrease of MDA and increase of GPx, SOD, CAT in PQ +
PDTC group compared with PQ group (P<0.05 or P<0.01) in the corresponding sacrifice dates. The activity of
NF-kappaB in lung tissue of PQ group significantly increased on the 1st, the 3rd, the 7th and 14th day compared with control and
PDTC group (P<0.01). There was a significant decrease in
NF-kappaB activity on the 1st, the 3 rd, the 7th day in PQ +
PDTC group compared with PQ group (P<0.05 or P<0.01). The MPO activity on the 14th day was (119.56 +/- 21.23) U/L, was lower than that of PQ group (P<0.05). Meanwhile, the content of Hyp in PQ group was significantly higher than control and
PDTC group on the 14th, the 28th and the 56th day (P<0.01), and its content was lower in PQ +
PDTC group on the 28th and the 56th day (0.89 +/- 0.05), (0.93 +/- 0.13) microg/mg compared with PQ group (P<0.01). The histological changes such as alveolitis and
fibrosis in PQ +
PDTC group were slighter than those in PQ group.
CONCLUSION: