Sanjoinine A isolated from Semen Zizyphi Spinosi protects against kainic acid-induced convulsions.

Abstract

These experiments were performed to know whether sanjoinine A, a component of the alkaloid fraction of Semen Zizyphi Spinosi, acts as an anti-convulsive agent in the kainic acid (KA)-induced experimental convulsion model and whether these effects are mediated by decreased intracellular calcium. Oral administration of sanjoinine A (4 and 8 mg/kg) increased the survival rate and latency of convulsion onset, and decreased the seizure scores and the weight loss induced by intraperitoneal (i.p.) injection of KA (50 mg/kg) in mice. In addition, sanjoinine A protected against neuronal damage and apoptosis in the hippocampus after KA administration, as analyzed by using immunohistochemistry and TUNEL assay. Sanjoinine A also significantly blocked seizure-form electroencephalogram alterations induced by KA. Moreover, in cultured rat neuronal cells, sanjoinine A inhibited KA-induced cell death, as measured by propidium iodide detection. Sanjoinine A also increased intracellular chloride and inhibited the elevation of intracellular calcium induced by KA. Sanjoinine A, therefore protects against KA-induced convulsions by increasing intracellular chloride and reducing intracellular calcium levels.

• Authors: Sung-Ryul Yoon, Young-Jun Jo, Shulong Yang, Yun-Bae Kim, Sang-Yoon Nam, Hyoung-Chun Kim, Jin Tae Hong, Ki-Wan Oh
• Journal: Archives of pharmacal research (Arch Pharm Res) Vol. 32 Issue 11 Pg. 1515-23 (Nov 2009) ISSN: 0253-6269 [Print] Korea (South)
• PMID: 20091264 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Alkaloids
• Anticonvulsants
• Chlorides
• Peptides, Cyclic
• Sanjoinine A
• Kainic Acid
• Calcium

Topics

• Administration, Oral
• Alkaloids (chemistry, isolation & purification)
• Animals
• Anticonvulsants (administration & dosage, isolation & purification, pharmacology)
• Apoptosis (drug effects)
• Calcium (metabolism)
• Cells, Cultured
• Chlorides (metabolism)
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Electroencephalography
• Hippocampus (drug effects, metabolism)
• Immunohistochemistry
• In Situ Nick-End Labeling
• Kainic Acid
• Male
• Mice
• Mice, Inbred ICR
• Neurons (drug effects)
• Peptides, Cyclic (administration & dosage, isolation & purification, pharmacology)
• Rats
• Rats, Sprague-Dawley
• Seizures (prevention & control)
• Ziziphus (chemistry)