An autopsy case of rhabdomyolysis related to vegetamin and genetic analysis of the rhabdomyolysis-associated genes.

Abstract

We report an autopsy case of a man who died 2 days after taking an overdose of vegetamin. The autopsy findings were as follows: the epidermis on the axillary fossa and the inguinal skin had become macerated. Skeletal muscle was discolored. Concentrations of urea nitrogen, creatinine and urine myoglobin were 1.95 g/day, 0.66 g/day and 1100 ng/mL, respectively. Immunohistochemically, myoglobin was strongly stained at the Bowman's capsule, and tubular lumen and epithelium. 8-OH-dG was strongly stained in renal tubular epithelium in which cell nuclei were strongly stained. ORP-150 was observed in intraglomerular cells and renal tubular epithelium. The concentrations of phenobarbital, promethazine and chlorpromazine ranged from therapeutic to toxic levels, from toxic to lethal levels and toxic level, respectively. His cause of death was considered to be vegetamin-induced rhabdomyolysis. In genetic analysis of this subject, there were two heterozygous silent mutations in the three hot-spot regions in the RYR1 gene. In the CPT II gene, the subject was found to be heterozygous for an amino acid substitution in exon 4, (1203)G>A causing a (368)Val>Ile amino acid substitution. There was no mutation in the VLCAD gene or CYP2C19 gene. The subject was heterozygous for CYP2D6*1 and CYP2D6*2.

• Authors: Aya Matsusue, Kenji Hara, Mitsuyoshi Kageura, Masayuki Kashiwagi, Wang Lu, Akiko Ishigami, Takako Gotohda, Itsuo Tokunaga, Akiyoshi Nisimura, Tomoko Sugimura, Shin-Ichi Kubo
• Journal: Journal of forensic and legal medicine (J Forensic Leg Med) Vol. 17 Issue 1 Pg. 46-9 (Jan 2010) ISSN: 1878-7487 [Electronic] England
• PMID: 20083051 (Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: 2009 Elsevier Ltd and Faculty of Forensic and Legal Medicine. Published by Elsevier Ltd. All rights reserved.

Chemical References

• Antipsychotic Agents
• Drug Combinations
• Ryanodine Receptor Calcium Release Channel
• vegetamin
• Carnitine O-Palmitoyltransferase
• Promethazine
• Chlorpromazine
• Phenobarbital

Topics

• Adult
• Amino Acid Substitution
• Antipsychotic Agents (blood, poisoning)
• Carnitine O-Palmitoyltransferase (genetics)
• Chlorpromazine (blood, poisoning)
• Drug Combinations
• Exons
• Forensic Genetics
• Forensic Pathology
• Genetic Predisposition to Disease
• Heterozygote
• Humans
• Male
• Muscle, Skeletal (pathology)
• Mutation
• Phenobarbital (blood, poisoning)
• Promethazine (blood)
• Rhabdomyolysis (chemically induced, genetics)
• Ryanodine Receptor Calcium Release Channel (genetics)
• Suicide