Abstract | OBJECTIVE: DESIGN: Prospective, randomized laboratory investigation. SETTING: University-affiliated laboratory. SUBJECTS: Adult female rats. INTERVENTIONS: MEASUREMENTS AND MAIN RESULTS: Intratracheal pretreatment of the transplantable left lung with the TIP peptide, but not with an inactive mutant TIP peptide, resulted in significantly improved oxygenation 24 hrs after transplantation. This treatment led to a significantly reduced neutrophil content in the lavage fluid. Both the effects on oxygenation and neutrophil infiltration were inhibited by the epithelial sodium channel blocker amiloride. The TIP peptide blunted reactive oxygen species production in pulmonary artery endothelial cells under hypoxia and reoxygenation and reduced reactive oxygen species content in the transplanted rat lungs in vivo. Ussing chamber experiments using monolayers of primary type II rat pneumocytes indicated that the primary site of action of the peptide was on the apical side of these cells. CONCLUSIONS:
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Authors | Jürg Hamacher, Uz Stammberger, Jeremie Roux, Sanjiv Kumar, Guang Yang, Chenling Xiong, Ralph A Schmid, Richard M Fakin, Trinad Chakraborty, Hamid M D Hossain, Jean-François Pittet, Albrecht Wendel, Stephen M Black, Rudolf Lucas |
Journal | Critical care medicine
(Crit Care Med)
Vol. 38
Issue 3
Pg. 871-8
(Mar 2010)
ISSN: 1530-0293 [Electronic] United States |
PMID | 20081530
(Publication Type: Journal Article, Research Support, N.I.H., Intramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Disaccharides
- Neuropeptides
- Reactive Oxygen Species
- Sodium Channel Blockers
- Tumor Necrosis Factor-alpha
- tuberoinfundibular peptide 39
- Superoxides
- N,N-diacetylchitobiose
- Amiloride
- Oxygen
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Topics |
- Alveolar Epithelial Cells
(drug effects, physiology)
- Amiloride
(pharmacology)
- Animals
- Disaccharides
(pharmacology)
- Endothelial Cells
(drug effects, physiology)
- Endothelium, Vascular
(drug effects, physiopathology)
- Lung
(blood supply)
- Lung Transplantation
(physiology)
- Membrane Potentials
(drug effects, physiology)
- Neuropeptides
(pharmacology)
- Oxygen
(physiology)
- Pulmonary Alveoli
(drug effects, physiopathology)
- Rats
- Reactive Oxygen Species
(metabolism)
- Reperfusion Injury
(physiopathology)
- Respiratory Function Tests
- Sheep
- Sodium Channel Blockers
(pharmacology)
- Superoxides
(metabolism)
- Tumor Necrosis Factor-alpha
(pharmacology)
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