Abstract |
In order to identify new protein markers modified in placental diseases, high-throughput analysis of proteins in the plasma of pregnant women was carried out for normal and pathological pregnancies ( Preeclampsia and/or Intra-Uterine Growth Restriction) using iTRAQ technology. We could identify 166 proteins that were modified (p<0.05) and the technique used allowed the detection of previously undetected factors, such as various members of the SERPINA clade. The modifications of two proteins ( C reactive protein and antichymotrypsin, SERPINA3) were validated on individual samples. Complement and coagulation cascades proteins were significantly enriched among modified protein clusters in the case of intra-uterine growth restriction (p<2.6.10(-11)). Several proteins were specifically enriched in isolated preeclampsia and depleted when preeclampsia was complicated by intra-uterine growth restriction. These findings suggest that the growth restricted foeto-placental unit is able to moderate some changes in maternal plasma composition. Overall, the use of iTRAQ technology, for the first time on this subject, enabled us to provide a new list of proteins modified in placental diseases, among which proteins expressed at a low level that were not accessible by other methods.
|
Authors | Jana Auer, Luc Camoin, François Guillonneau, Virginie Rigourd, Sonia T Chelbi, Marjorie Leduc, Jérôme Laparre, Thérèse-Marie Mignot, Daniel Vaiman |
Journal | Journal of proteomics
(J Proteomics)
Vol. 73
Issue 5
Pg. 1004-17
(Mar 10 2010)
ISSN: 1876-7737 [Electronic] Netherlands |
PMID | 20079470
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright (c) 2010 Elsevier B.V. All rights reserved. |
Chemical References |
- Biomarkers
- Blood Proteins
- SERPINA3 protein, human
- Serpins
- C-Reactive Protein
|
Topics |
- Biomarkers
- Blood Proteins
(analysis)
- C-Reactive Protein
(analysis)
- Case-Control Studies
- Female
- Fetal Growth Retardation
(blood, diagnosis)
- Humans
- Pre-Eclampsia
(blood, diagnosis)
- Pregnancy
- Serpins
(blood)
|