[D9 measles virus was first isolated from an imported measles case in Sichuan Province of China].

Abstract

OBJECTIVE:

To isolate and identify the genotype and molecular characteristic of an imported D9 measles virus.

METHOD:

To isolate the virus with Vero/SLAM cell line. And RT-PCR (reverse transcription-polymerase chain reaction) were performed to amplify the 450 nucleotide acids of carboxyl terminal of N (nucleoprotein, N) protein. The phylogenetic tree was constructed and the homology similarity was analyzed.

RESULTS:

Sichuan isolate MVi/Sichuan.CHN/07.09/1 was clustered within the same genotype group with WHO D9 genotype reference strain, the homology of nucleotide acid between Sichuan isolate and WHO D9 genotype reference strain was 96.9%. The homology of nucleotide acid and amino acid between Sichuan isolate and 2008 D9 genotype representative strain were 99.8%-100% and 99.3%-100% respectively. Compared with the Chinese measles vaccine strain, the homology of nucleotide acid and amino acid of Shanghai-191 were 92.3% and 90.7% respectively. Compared with the endemic H1 genotype representative measles strain, the homology of nucleotide acid and amino acid were 90.8% and 92.1% respectively.

CONCLUSION:

The imported virus was D9 genotype measles virus.

• Authors: Yan Zhang, Ji-Lan He, Li Sun
• Journal: Zhongguo yi miao he mian yi (Zhongguo Yi Miao He Mian Yi) Vol. 15 Issue 4 Pg. 304-9 (Aug 2009) ISSN: 1006-916X [Print] China
• PMID: 20077726 (Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Animals
• China
• Chlorocebus aethiops
• Female
• Genotype
• Humans
• Measles (virology)
• Measles virus (classification, genetics, isolation & purification)
• Molecular Sequence Data
• Phylogeny
• Travel
• Vero Cells