Abstract | BACKGROUND: Endoscopy capable of fluorescence observation provides histological information on gastrointestinal lesions. We explored the novel application of low pH-dependent fluorescent dyes for fluorescence observation of crypt structure and inflammatory cell infiltration in the colon. METHODS: RESULTS: Topical application of low pH-dependent fluorescent dyes revealed crypts as ring-shaped fluorescent stains by visualizing the mucin granules of goblet cells. Because of the minimal fluorescence intensity of the low pH-dependent fluorescent dyes in phosphate-buffered saline, it was not necessary to wash the mucosa before the fluorescence observation. 4-Nitro-7-piperazino-2,1,3-benzoxadiazole ( NBD-PZ) was quicker to achieve complete staining (three minutes) than LysoSensor Green DND-153 and DND-189 (20 minutes). In each type of colitis, NBD-PZ revealed the destruction of the crypts as the disappearance of the ring-shaped fluorescent stains and the infiltration of inflammatory cells as the aggregation of punctate fluorescent stains through visualization of lysosomes. CONCLUSIONS: Low pH-dependent fluorescent dyes, especially NBD-PZ, are suitable for topical application to the colonic mucosa and have characteristics that allow for the histological examination of colitis.
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Authors | Kazuhiro Ishiguro, Takafumi Ando, Osamu Watanabe, Hidemi Goto |
Journal | BMC gastroenterology
(BMC Gastroenterol)
Vol. 10
Pg. 4
(Jan 15 2010)
ISSN: 1471-230X [Electronic] England |
PMID | 20074376
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- 4-nitro-7-piperazino-2,1,3-benzoxadiazole
- Fluorescent Dyes
- Macrolides
- Oxadiazoles
- Piperazines
- Interleukin-10
- bafilomycin A1
- Proton-Translocating ATPases
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Topics |
- Administration, Rectal
- Animals
- Colitis
(drug therapy, pathology)
- Fluorescent Dyes
(administration & dosage, analysis)
- Hydrogen-Ion Concentration
- Interleukin-10
(deficiency)
- Intestinal Mucosa
(pathology)
- Leukocytes, Mononuclear
(metabolism, pathology)
- Macrolides
(therapeutic use)
- Mice
- Mice, Inbred BALB C
- Microscopy, Fluorescence
- Oxadiazoles
(administration & dosage, analysis)
- Piperazines
(administration & dosage, analysis)
- Proton-Translocating ATPases
(antagonists & inhibitors)
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