Abstract |
The immunodominance of Mycobacterium tuberculosis proteins malate synthase (MS) and MPT51 has been demonstrated in case-control studies with patients from countries in which tuberculosis (TB) is endemic. The value of these antigens for the serodiagnosis of TB now is evaluated in a cross-sectional study of pulmonary TB suspects in the United States diagnosed to have TB, HIV-associated TB, or other respiratory diseases (ORD). Serum antibody reactivity to recombinant purified MS and MPT51 was determined by enzyme-linked immunosorbent assays (ELISAs) of samples from TB suspects and well-characterized control groups. TB suspects were diagnosed with TB (n = 87; 49% sputum microscopy negative, 20% HIV(+)) or ORD (n = 63; 58% HIV(+)). Antibody reactivity to MS and MPT51 was significantly higher in U.S. HIV(+)/TB samples than in HIV(-)/TB samples (P < 0.001), and it was significantly higher in both TB groups than in control groups with latent TB infection (P < 0.001). Antibody reactivity to both antigens was higher in U.S. HIV(+)/TB samples than in HIV(+)/ORD samples (P = 0.052 for MS, P = 0.001 for MPT51) but not significantly different between HIV(-)/TB and HIV(-)/ORD. Among U.S. HIV(+) TB suspects, a positive anti-MPT51 antibody response was strongly and significantly associated with TB (odds ratio, 11.0; 95% confidence interval, 2.3 to 51.2; P = 0.002). These findings have implications for the adjunctive use of TB serodiagnosis with these antigens in HIV(+) subjects.
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Authors | Jacqueline M Achkar, Elisabeth Jenny-Avital, Xian Yu, Susanne Burger, Eric Leibert, Patrick W Bilder, Steven C Almo, Arturo Casadevall, Suman Laal |
Journal | Clinical and vaccine immunology : CVI
(Clin Vaccine Immunol)
Vol. 17
Issue 3
Pg. 384-92
(Mar 2010)
ISSN: 1556-679X [Electronic] United States |
PMID | 20071491
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Antibodies, Bacterial
- Antigens, Bacterial
- Bacterial Proteins
- Immunodominant Epitopes
- MPT51 protein, Mycobacterium
- Recombinant Proteins
- Malate Synthase
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Topics |
- Adult
- Antibodies, Bacterial
(blood)
- Antigens, Bacterial
(immunology)
- Bacterial Proteins
(immunology)
- Cross-Sectional Studies
- Enzyme-Linked Immunosorbent Assay
- Female
- HIV Infections
(complications)
- Humans
- Immunodominant Epitopes
(immunology)
- Latent Tuberculosis
(complications, diagnosis, immunology)
- Malate Synthase
(immunology)
- Male
- Middle Aged
- Mycobacterium tuberculosis
(immunology)
- Recombinant Proteins
(immunology)
- Tuberculosis, Pulmonary
(complications, diagnosis, immunology)
- United States
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