Pisosterol induces interphase arrest in HL60 cells with c-MYC amplification.
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| Abstract |
The leukaemia cell line HL60 is widely used in studies of the cell cycle, apoptosis and adhesion mechanisms in cancer cells. One marked characteristic of HL60 cells is the c-MYC proto-oncogene amplification, resulting in the formation of homogeneously staining regions (HSRs) at 8p24. We conducted a fluorescence in situ hybridization study in an HL60 cell line, using a locus-specific probe for c-MYC, before and after treatment with pisosterol (at 0.5, 1.0 and 1.8 microg/mL), a triterpene isolated from the fungus Pisolithus tinctorius. Before treatment, 87.5% of the cells showed HSRs. After treatment, no effects were detected at lower concentrations of pisosterol (0.5 and 1.0 microg/mL). However, at 1.8 microg/mL only 15% of the cells presented HSRs, and 39.5% presented few fluorescent signals (3 or 4 alleles), suggesting that pisosterol probably blocks the cells with HSRs at interphase. This result is particularly interesting because cells that do not show a high degree of c-MYC gene amplification have a less aggressive and invasive behaviour and are easy targets for chemotherapy. Therefore, further studies are needed to examine the use of pisosterol in combination with conventional anti-cancer therapy.
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| Authors | T C R Silva, P D L Lima, M O Bahia, A S Khayat, F S Bezerra, M Andrade-Neto, A D Seabra, T B Pontes, M O Moraes, R C Montenegro, L V Costa-Lotufo, C Pessoa, G R Pinto, R R Burbano |
| Journal | Human & experimental toxicology (Hum Exp Toxicol) Vol. 29 Issue 3 Pg. 235-40 (Mar 2010) ISSN: 1477-0903 [Electronic] England |
| PMID | 20071475 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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