Abstract |
The objective of our studies is the development of a novel formulation of nystatin (NYT) that could be administered systemically and might be used for therapy of invasive mycoses. We developed a formulation of nystatin and intralipid (IL), which is a clinically used food supplement, and this report focuses on the characterization of NYT-IL, assessment of its antifungal activity and in vitro toxicity. We characterized physical properties of the NYT-IL preparation and its stability during storage. Susceptibility of Candida, Aspergillus and Fusarium species was determined using a CLSI technique. In vitro toxicity of NYT-IL was assessed using an assay measuring hemolysis of sheep red blood cells (SRBC) and leakage of potassium. It was found that: (1) the particle size in NYT-IL did not differ from that of IL; (2) over 80% of NYT was in association with IL; and (3) these features did not change during storage. All Candida and Aspergillus strains had lower minimal inhibitory concentration (MIC) values for NYT-IL than that for NYT; the MICs of the Fusarium strains were similar for NYT & NYT-IL. Toxicity assays showed that the NYT-IL formulation is less toxic than NYT. In conclusion, we describe a novel, characterized, stable formulation of nystatin, nystatin- intralipid, with in vitro activity against pathogenic Candida and Aspergillus species.
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Authors | R Semis, I Polacheck, E Segal |
Journal | Mycopathologia
(Mycopathologia)
Vol. 169
Issue 5
Pg. 333-41
(May 2010)
ISSN: 1573-0832 [Electronic] Netherlands |
PMID | 20069459
(Publication Type: Journal Article)
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Chemical References |
- Antifungal Agents
- Fat Emulsions, Intravenous
- Nystatin
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Topics |
- Animals
- Antifungal Agents
(chemistry, pharmacology)
- Aspergillus
(drug effects)
- Candida
(drug effects)
- Dietary Supplements
- Erythrocytes
(drug effects)
- Fat Emulsions, Intravenous
(chemistry, pharmacology)
- Fusarium
(drug effects)
- Humans
- Microbial Sensitivity Tests
- Nystatin
(chemistry, pharmacology)
- Sheep
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